PUBLICATION
Effects of water hardness, alkalinity, and dissolved organic carbon on the toxicity of copper to the lateral line of developing fish
- Authors
- Linbo, T.L., Baldwin, D.H., McIntyre, J.K., and Scholz, N.L.
- ID
- ZDB-PUB-090217-37
- Date
- 2009
- Source
- Environmental toxicology and chemistry 28(7): 1455-1461 (Journal)
- Registered Authors
- Baldwin, David, Linbo, Tiffany L., Scholz, Nat
- Keywords
- Zebrafish, Hair cells, Mechanosensory, Bioavailability, Biotic ligand model
- MeSH Terms
-
- Animals
- Copper/toxicity*
- Fresh Water/chemistry*
- Gills/drug effects
- Humic Substances/analysis*
- Larva/drug effects
- Lateral Line System/drug effects*
- Lateral Line System/growth & development
- Mechanoreceptors/drug effects
- Neurons/drug effects
- Toxicity Tests
- Water Pollutants, Chemical/toxicity*
- Zebrafish/growth & development*
- PubMed
- 19215183 Full text @ Environ. Toxicol. Chem.
Citation
Linbo, T.L., Baldwin, D.H., McIntyre, J.K., and Scholz, N.L. (2009) Effects of water hardness, alkalinity, and dissolved organic carbon on the toxicity of copper to the lateral line of developing fish. Environmental toxicology and chemistry. 28(7):1455-1461.
Abstract
Conventional water chemistry parameters such as hardness, alkalinity, and organic carbon are known to affect the acutely lethal toxicity of copper to fish and other aquatic organisms. In the present study, we investigate the influence of these water chemistry parameters on short-term (3 h), sublethal (0 - 40 microg/L) copper toxicity to the peripheral mechanosensory system of larval zebrafish (Danio rerio) using an in vivo fluorescent marker of lateral line sensory neuron (hair cell) integrity. We studied the influence of hardness (via CaCl2, MgSO4, or both at a 2:1 molar ratio), sodium (via NaHCO3 or NaCl), and organic carbon on copper-induced neurotoxicity to zebrafish lateral line neurons over a range of environmentally relevant water chemistries. For all water parameters but organic carbon, the reductions in copper toxicity, while statistically significant, were small. Increasing organic carbon across a range of environmentally relevant concentrations (0.1 - 4.3 mg/L) increased the EC50 for copper toxicity (the effective concentration resulting in a 50% loss of hair cells) from approximately 12 microg/L to approximately 50 microg/L. Finally, we used an ionoregulatory-based biotic ligand model to compare copper toxicity mediated by targets in the fish gill and lateral line. Relative to copper toxicity via the gill, we find that individual water chemistry parameters are less influential in terms of reducing cytotoxic impacts to the mechanosensory system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping