PUBLICATION
Pseudomonas aeruginosa Type III secretion system interacts with phagocytes to modulate systemic infection of zebrafish embryos
- Authors
- Brannon, M.K., Davis, J.M., Mathias, J.R., Hall, C.J., Emerson, J.C., Crosier, P.S., Huttenlocher, A., Ramakrishnan, L., and Moskowitz, S.M.
- ID
- ZDB-PUB-090217-28
- Date
- 2009
- Source
- Cellular Microbiology 11(5): 755-768 (Journal)
- Registered Authors
- Crosier, Phil, Davis, James M., Hall, Chris, Huttenlocher, Anna, Mathias, Jonathan, Ramakrishnan, Lalita
- Keywords
- none
- MeSH Terms
-
- Animals
- Bacterial Secretion Systems/genetics
- Bacterial Secretion Systems/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/microbiology*
- Immunity, Innate
- Macrophages/cytology
- Macrophages/microbiology
- Macrophages/physiology
- Models, Animal
- Neutrophils/cytology
- Neutrophils/microbiology
- Neutrophils/physiology
- Phagocytes/cytology
- Phagocytes/microbiology
- Phagocytes/physiology*
- Phagocytosis
- Pseudomonas aeruginosa/genetics
- Pseudomonas aeruginosa/pathogenicity*
- Virulence
- Zebrafish/embryology
- Zebrafish/microbiology*
- PubMed
- 19207728 Full text @ Cell. Microbiol.
Citation
Brannon, M.K., Davis, J.M., Mathias, J.R., Hall, C.J., Emerson, J.C., Crosier, P.S., Huttenlocher, A., Ramakrishnan, L., and Moskowitz, S.M. (2009) Pseudomonas aeruginosa Type III secretion system interacts with phagocytes to modulate systemic infection of zebrafish embryos. Cellular Microbiology. 11(5):755-768.
Abstract
Summary Pseudomonas aeruginosa is an opportunistic human pathogen that can cause serious infection in those with deficient or impaired phagocytes. We have developed the optically transparent and genetically tractable zebrafish embryo as a model for systemic P. aeruginosa infection. Despite lacking adaptive immunity at this developmental stage, zebrafish embryos were highly resistant to P. aeruginosa infection, but as in humans, phagocyte depletion dramatically increased their susceptibility. The virulence of an attenuated P. aeruginosa strain lacking a functional Type III secretion system was restored upon phagocyte depletion, suggesting that this system influences virulence through its effects on phagocytes. Intravital imaging revealed bacterial interactions with multiple blood cell types. Neutrophils and macrophages rapidly phagocytosed and killed P. aeruginosa, suggesting that both cell types play a role in protection against infection. Intravascular aggregation of erythrocytes and other blood cells with resultant circulatory blockage was observed immediately upon infection, which may be relevant to the pathogenesis of thrombotic complications of human P. aeruginosa infections. The real-time visualization capabilities and genetic tractability of the zebrafish infection model should enable elucidation of molecular and cellular details of P. aeruginosa pathogenesis in conditions associated with neutropenia or impaired phagocyte function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping