Cytochrome P450 1D1: A novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD
- Goldstone, J.V., Jönsson, M.E., Behrendt, L., Woodin, B.R., Jenny, M.J., Nelson, D.R., and Stegeman, J.J.
- Archives of biochemistry and biophysics 482(1-2): 7-16 (Journal)
- Registered Authors
- Goldstone, Jed, Stegeman, John J.
- Development, CYP1A, CYP1B, CYP1C, AHR, PCB126, TCDD, Zebrafish, Embryo, Vertebrate toxicology, Oxidative biotransformation
- MeSH Terms
- Cloning, Molecular
- Cytochrome P-450 CYP1A1/genetics
- Cytochrome P-450 Enzyme System/genetics*
- DNA Primers
- Gene Amplification
- Gene Expression Regulation/drug effects*
- Polychlorinated Biphenyls/pharmacology*
- Polymerase Chain Reaction
- RNA, Messenger/genetics
- RNA-Directed DNA Polymerase
- Transcription, Genetic*
- Zebrafish Proteins/genetics*
- 19103147 Full text @ Arch. Biochem. Biophys.
Goldstone, J.V., Jönsson, M.E., Behrendt, L., Woodin, B.R., Jenny, M.J., Nelson, D.R., and Stegeman, J.J. (2009) Cytochrome P450 1D1: A novel CYP1A-related gene that is not transcriptionally activated by PCB126 or TCDD. Archives of biochemistry and biophysics. 482(1-2):7-16.
Enzymes in the cytochrome P450 1 family oxidize many common environmental toxicants. We identified a new CYP1, termed CYP1D1, in zebrafish. Phylogenetically, CYP1D1 is paralogous to CYP1A and the two share 45% amino acid identity and similar gene structure. In adult zebrafish, CYP1D1 is most highly expressed in liver and is relatively highly expressed in brain. CYP1D1 transcript levels were higher at 9h post-fertilization than at later developmental times. Treatment of zebrafish with potent aryl hydrocarbon receptor (AHR) agonists (3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin) did not induce CYP1D1 transcript expression. Morpholino oligonucleotide knockdown of AHR2, which mediates induction of other CYP1s, did not affect CYP1D1 expression. Zebrafish CYP1D1 heterologously expressed in yeast exhibited ethoxyresorufin- and methoxyresorufin-O-dealkylase activities. Antibodies against a CYP1D1 peptide specifically detected a single electrophoretically-resolved protein band in zebrafish liver microsomes, distinct from CYP1A. CYP1D1 in zebrafish is a CYP1A-like gene that could have metabolic functions targeting endogenous compounds.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes