PUBLICATION

Expression screening and annotation of a zebrafish myoblast cDNA library

Authors
Baxendale, S., Chen, C.K., Tang, H., Davison, C., Hateren, L.V., Croning, M.D., Humphray, S.J., Hubbard, S.J., and Ingham, P.W.
ID
ZDB-PUB-081121-5
Date
2009
Source
Gene expression patterns : GEP   9(2): 73-82 (Journal)
Registered Authors
Baxendale, Sarah, Davison, Claire, Humphray, Sean, Ingham, Philip
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian
  • Expressed Sequence Tags
  • Gene Expression Profiling/methods*
  • Gene Library*
  • Genomics/methods
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Myoblasts/metabolism*
  • Organ Specificity/genetics
  • Sequence Analysis, DNA/methods*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
19007914 Full text @ Gene Expr. Patterns
Abstract
To analyse the myogenic transcriptome and identify novel genes involved in muscle development in an in vivo context, we have constructed a muscle specific cDNA library from GFP-expressing myoblasts purified by fluorescent activated cell sorting of transgenic zebrafish embryos. We have generated 153,428 EST sequences from this library that have been clustered into consensi, mapped to the genome assembly Zv6 and analysed for protein homology. Expression analysis of a randomly picked sample of clones using whole mount in situ hybridisation, identified 30 genes that are expressed specifically within the myotome, one third of which represent novel sequences. These genes have been assigned to syn-expression groups. The sequencing of the myoblast enriched cDNA library has significantly increased the number of zebrafish ESTs, facilitating the prediction of new spliced transcripts in the genome assembly and providing a transcriptome of an in vivo myoblast cell.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes