PUBLICATION

Maintenance of thymic epithelial phenotype requires extrinsic signals in mouse and zebrafish

Authors
Soza-Ried, C., Bleul, C.C., Schorpp, M., and Boehm, T.
ID
ZDB-PUB-081008-9
Date
2008
Source
Journal of immunology (Baltimore, Md. : 1950)   181(8): 5272-5277 (Journal)
Registered Authors
Boehm, Tom, Schorpp, Michael
Keywords
none
MeSH Terms
  • Animals
  • Biological Evolution
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/immunology
  • Bone Morphogenetic Proteins/metabolism*
  • Epithelium/embryology*
  • Epithelium/immunology
  • Forkhead Transcription Factors/biosynthesis*
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/immunology
  • Gene Expression Regulation, Developmental/physiology
  • Integrases/genetics
  • Integrases/immunology
  • Integrases/metabolism
  • Mice
  • Mice, Transgenic
  • Pharynx/embryology
  • Pharynx/immunology
  • Signal Transduction/genetics
  • Signal Transduction/immunology*
  • T-Lymphocytes/immunology
  • T-Lymphocytes/metabolism
  • Thymus Gland/embryology*
  • Thymus Gland/immunology
  • Transgenes/immunology
  • Zebrafish
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
PubMed
18832682 Full text @ J. Immunol.
Abstract
Thymopoiesis strictly depends on proper differentiation of the thymic epithelial anlage. Differentiation of thymic epithelial cells (TECs) is controlled by the Foxn1 transcription factor. The in vivo signals initiating and maintaining Foxn1 expression in the future thymus anlage are unknown. In the mouse, bone morphogenetic protein (BMP) signaling is required for the maintenance of Foxn1 expression in TECs, as shown here by lineage tracing using a Foxn1-driven Cre transgene. Loss of Foxn1 expression after BMP inhibition reverts TECs to a basal state of pharyngeal epithelium unable to support T cell development; it does not divert them into a parathyroid fate. In zebrafish larvae, BMP inhibition likewise causes loss of foxn1 expression in the thymic anlage and subsequent impairment of thymopoiesis. These results indicate an evolutionarily conserved role of BMP signaling in the maintenance of Foxn1 expression.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes