PUBLICATION
Graded levels of Ptf1a differentially regulate endocrine and exocrine fates in the developing pancreas
- Authors
- Dong, P.D., Provost, E., Leach, S.D., and Stainier, D.Y.
- ID
- ZDB-PUB-081007-1
- Date
- 2008
- Source
- Genes & Development 22(11): 1445-1450 (Journal)
- Registered Authors
- Dong, P. Duc, Leach, Steven D., Stainier, Didier
- Keywords
- Ptf1a, diabetes, endocrine, exocrine, pancreas
- MeSH Terms
-
- Gene Expression Regulation, Developmental*
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Pancreas/embryology*
- Pancreas/metabolism
- Animals
- Pancreas, Exocrine/embryology*
- Islets of Langerhans/embryology*
- Transcription Factors/genetics
- PubMed
- 18519637 Full text @ Genes & Dev.
Citation
Dong, P.D., Provost, E., Leach, S.D., and Stainier, D.Y. (2008) Graded levels of Ptf1a differentially regulate endocrine and exocrine fates in the developing pancreas. Genes & Development. 22(11):1445-1450.
Abstract
The mechanisms regulating pancreatic endocrine versus exocrine fate are not well defined. By analyzing the effects of Ptf1a partial loss of function, we uncovered novel roles for this transcription factor in determining pancreatic fates. In a newly identified hypomorphic ptf1a mutant, pancreatic cells that would normally express ptf1a and become exocrine cells, express the endocrine marker Isl1, indicating a cell fate switch. Surprisingly, a milder reduction of Ptf1a leads to an even greater increase of ectopic endocrine cells, suggesting that Ptf1a also plays a role in promoting endocrine development. We propose that low levels of Ptf1a promote endocrine fate, whereas high levels repress endocrine fate and promote exocrine fate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping