ZFIN ID: ZDB-PUB-080902-27
In vivo birthdating by BAPTISM reveals that trigeminal sensory neuron diversity depends on early neurogenesis
Caron, S.J., Prober, D., Choy, M., and Schier, A.F.
Date: 2008
Source: Development (Cambridge, England)   135(19): 3259-3269 (Journal)
Registered Authors: Caron, Sophie, Choy, Margaret, Prober, David, Schier, Alexander
Keywords: Neurogenesis, Trigeminal sensory ganglia, Trp, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Gene Expression Regulation, Developmental
  • Ion Channels/genetics
  • Ion Channels/metabolism
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Models, Neurological
  • Neurogenesis*/genetics
  • Neurogenesis*/physiology
  • Recombinant Proteins/genetics
  • Recombinant Proteins/metabolism
  • Sensory Receptor Cells/cytology*
  • Sensory Receptor Cells/metabolism
  • Time Factors
  • Trigeminal Ganglion/cytology
  • Trigeminal Ganglion/embryology*
  • Trigeminal Ganglion/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 18755773 Full text @ Development
Among sensory systems, the somatic sense is exceptional in its ability to detect a wide range of chemical, mechanical and thermal stimuli. How this sensory diversity is established during development remains largely elusive. We devised a method (BAPTISM) that uses the photoconvertible fluorescent protein Kaede to simultaneously analyze birthdate and cell fate in live zebrafish embryos. We found that trigeminal sensory ganglia are formed from early-born and late-born neurons. Early-born neurons give rise to multiple classes of sensory neurons that express different ion channels. By contrast, late-born neurons are restricted in their fate and do not form chemosensory neurons expressing the ion channel TrpA1b. Accordingly, larvae lacking early-born neurons do not respond to the TrpA1b agonist allyl isothiocyanate. These results indicate that the multimodal specification and function of trigeminal sensory ganglia depends on the timing of neurogenesis.