PUBLICATION
Expression and Regulation of the Vitamin D Receptor in the Zebrafish, Danio rerio
- Authors
- Craig, T.A., Sommer, S., Sussman, C.R., Grande, J.P., and Kumar, R.
- ID
- ZDB-PUB-080421-1
- Date
- 2008
- Source
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 23(9): 1486-1496 (Journal)
- Registered Authors
- Sussman, Caroline R.
- Keywords
- Zebrafish, Vitamin D Receptor, 1α,25-dihydroxyvitamin D, calcium
- MeSH Terms
-
- 24,25-Dihydroxyvitamin D 3/pharmacology
- Animals
- Antibodies/pharmacology
- Cryoultramicrotomy
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Eye/cytology
- Eye/drug effects
- Eye/metabolism
- Female
- Fertilization/drug effects
- Immune Sera
- Intestines/drug effects
- Intestines/metabolism
- Lithocholic Acid/pharmacology
- Male
- Nervous System/cytology
- Nervous System/drug effects
- Nervous System/metabolism
- Organ Specificity/drug effects
- Receptors, Calcitriol/metabolism*
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 18410235 Full text @ J. Bone Miner. Res.
- CTD
- 18410235
Citation
Craig, T.A., Sommer, S., Sussman, C.R., Grande, J.P., and Kumar, R. (2008) Expression and Regulation of the Vitamin D Receptor in the Zebrafish, Danio rerio. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 23(9):1486-1496.
Abstract
Vitamin D and vitamin D metabolites such as 25-hydroxyvitamin D and 1alpha, 25-dihydroxyvitamin D (1alpha, 25(OH)(2)D(3)) circulate in the serum of fish. The receptor for 1alpha, 25(OH)(2)D(3) (VDR) has previously been cloned from fish intestine, and ligand binding assays have demonstrated the presence of the VDR in the gills, intestine and liver of fish. Using immunohistochemical methods with specific antibodies against the VDR, we now report that the VDR is widely expressed in tissues of the adult male and female zebrafish, Danio rerio, specifically in epithelial cells of gills, tubular cells of the kidney, and in absorptive cells in the intestine. Additionally, the VDR is expressed in the skin, the olfactory organ, in the retina, brain, and spinal cord. Sertoli cells of the testis, oocytes, acinar cells of the pancreas, hepatocytes and bile duct epithelial cells express substantial amounts of the receptor. Osteoblast-like cells and chondrocytes also express the VDR. Pre-immune serum and anti-serum pre-adsorbed with Danio VDR protein fails to detect the VDR in the same tissues. The VDR is also present in the developing eye, brain, and otic vesicle of 48 h and 96h post-fertilization zebrafish embryos. Parenteral administration of 1alpha, 25(OH)(2)D(3) increases concentrations of the VDR in intestinal epithelial cells but not in epithelial cells of the gills. Lithocholic acid, however, does not alter concentrations of the VDR following parenteral administration. The data suggest that the VDR is widely distributed in tissues of the zebrafish, Danio rerio, and is likely to play important roles in epithelial transport, bone and endocrine function. Furthermore, concentrations of the receptor appear to be regulated by its ligand, 1alpha, 25-dihydroxyvitamin D, but not by lithocholic acid. Zebrafish may serve as a useful model in which to assess the function of the VDR in diverse tissues.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping