|ZFIN ID: ZDB-PUB-080327-8|
Identification and characterization of zebrafish ocular formation genes
Wang, H., Kesinger, J.W., Zhou, Q., Wren, J.D., Martin, G., Turner, S., Tang, Y., Frank, M.B., and Centola, M.
|Source:||Genome 51(3): 222-235 (Journal)|
|Registered Authors:||Wang, Han|
|Keywords:||masterblind/axin1, retina, lens, opsin, crystallin, bzw1a, atp6v0c, hspa8, rpl7a, oligonucleotide microarrays, in situ hybridization|
|PubMed:||18356958 Full text @ Genome|
Wang, H., Kesinger, J.W., Zhou, Q., Wren, J.D., Martin, G., Turner, S., Tang, Y., Frank, M.B., and Centola, M. (2008) Identification and characterization of zebrafish ocular formation genes. Genome. 51(3):222-235.
ABSTRACTTo study genes that are specifically expressed in the eyes, we employed microarray and in situ hybridization analyses to identify and characterize differentially expressed ocular genes in eyeless masterblind (mbl−/−) zebrafish (Danio rerio). Among 70 differentially expressed genes in the mbl-/- mutant identified by microarray analysis, 8 down-regulated genes were characterized, including 4 eye-specific genes, opsin 1 short-wave-sensitive 1 (opn1sw1), crystallinssa1b (cryba1b), crystallinssa2b (cryba2b), and crystallinγ M2d3 (crygm2d3); 2 eye and brain genes, ATPase, H+ transporting, lysosomal, V0 subunit c (atp6v0c) and basic leucine zipper and W2 domains 1a (bzw1a)nssa1b (cryba1b), crystallinssa2b (cryba2b), and crystallinγ M2d3 (crygm2d3); 2 eye and brain genes, ATPase, H+ tra; and 2 constitutive genes, heat shock protein 8 (hspa8) and ribosomal protein L7a (rpl7a). In situ hybridization experiments confirmed down-regulation of these 8 ocular formation genes in mbl-/- zebrafish and showed their ocular and dynamic temporal expression patterns during zebrafish early development. Further, an automated literature analysis of the 70 differentially expressed genes identified a sub-network of genes with known associations, either with each other or with ocular structures or development, and shows how this study contributes to the current body of knowledge.