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Research
General Information
ZIRC
ZFIN ID: ZDB-PERS-981201-1
Wang, Han
Email: han.wang88@gmail.com
URL:
Affiliation: The Wang Laboratory
Address: Professor and Director Center for Circadian Clocks Soochow University 199 Renai Road Suzhou, Jiangsu, 215123 China
Country: China
Phone: 8651265882115
Fax: 8651265882115
Orcid ID: 0000-0003-2420-3147


BIOGRAPHY AND RESEARCH INTERESTS
Most organisms display daily changes at molecular, cellular, physiological, and behavioral levels, which are known as circadian rhythms. The circadian clock controls fundamental life processes such as the human sleep-wake cycle. Many human sleep disorders and dysrhythmias are thought to result from circadian defects. A full molecular genetic understanding of circadian rhythmicity is critical for diagnosis, prevention and treatment of human dysrhythmias. We study molecular genetic mechanisms underlying zebrafish circadian rhythmicity. Using TALEN and CRISPR/Cas9 techniques, we have generated a number of mutants for zebrafish circadian clock genes. The roles of these circadian clock genes in the circadian system and life processes are investigated.

We also used zebrafish to study a group of human diseases called porphyria, derived from defective enzymes in heme biosynthesis. We have established several zebrafish models for human porphyria. We are interested in studying novel aspects of porphyria pathogenesis.


PUBLICATIONS
Zheng, Y., Liu, C., Li, Y., Jiang, H., Yang, P., Tang, J., Xu, Y., Wang, H., He, Y. (2018) Loss-of-function mutations with circadian rhythm regulator Per1/Per2 lead to premature ovarian insufficiency. Biology of reproduction. 100(4):1066-1072
Huang, D.F., Wang, M.Y., Yin, W., Ma, Y.Q., Wang, H., Xue, T., Ren, D.L., Hu, B. (2018) Zebrafish Lacking Circadian Gene per2 Exhibit Visual Function Deficiency.. Frontiers in behavioral neuroscience. 12:53
Zhong, Y., Ye, Q., Chen, C., Wang, M., Wang, H. (2018) Ezh2 promotes clock function and hematopoiesis independent of histone methyltransferase activity in zebrafish. Nucleic acids research. 46(7):3382-3399
Nie, K., Wang, K., Huang, D.F., Huang, Y.B., Yin, W., Ren, D.L., Wang, H., Hu, B. (2017) Effects of circadian clock protein Per1b on zebrafish visual functions. Chronobiology International. 35(2):160-168
Han, J., Ji, C., Guo, Y., Yan, R., Hong, T., Dou, Y., An, Y., Tao, S., Qin, F., Nie, J., Ji, C., Wang, H., Tong, J., Xiao, W., Zhang, J. (2017) Mechanisms underlying melatonin-mediated prevention of fenvalerate-induced behavioral and oxidative toxicity in zebrafish. Journal of toxicology and environmental health. Part A. 80(23-24):1331-1341
Ren, D.L., Ji, C., Wang, X.B., Wang, H., Hu, B. (2017) Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish. Scientific Reports. 7:4696
Xiao, B., Cui, L.Q., Ding, C., Wang, H. (2017) Effects of Lithium and 2,4-Dichlorophenol on Zebrafish: Circadian Rhythm Disorder and Molecular Effects. Zebrafish. 14(3):209-215
Wang, Y., Liu, W., Yang, J., Wang, F., Yizhen, S., Zhong, Z.M., Wang, H., Hu, L.F., Liu, C.F. (2017) Parkinson's disease-like motor and non-motor symptoms in rotenone-treated zebrafish. Neurotoxicology. 58:103-109
Pei, S., Liu, L., Zhong, Z., Wang, H., Lin, S., Shang, J. (2016) Risk of prenatal depression and stress treatment: alteration on serotonin system of offspring through exposure to Fluoxetine. Scientific Reports. 6:33822
Huang, G., Zhang, F., Ye, Q., Wang, H. (2016) The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erbα and indirectly via Cebpb/(C/ebpβ) in zebrafish. Autophagy. 12(8):1292-309
Bo, L., Liu, Z., Zhong, Y., Huang, J., Chen, B., Wang, H., Xu, Y. (2016) Iron deficiency anemia's affect on bone formation in zebrafish mutant. Biochemical and Biophysical Research Communications. 475(3):271-6
Braasch, I., Gehrke, A.R., Smith, J.J., Kawasaki, K., Manousaki, T., Pasquier, J., Amores, A., Desvignes, T., Batzel, P., Catchen, J., Berlin, A.M., Campbell, M.S., Barrell, D., Martin, K.J., Mulley, J.F., Ravi, V., Lee, A.P., Nakamura, T., Chalopin, D., Fan, S., Wcisel, D., Cañestro, C., Sydes, J., Beaudry, F.E., Sun, Y., Hertel, J., Beam, M.J., Fasold, M., Ishiyama, M., Johnson, J., Kehr, S., Lara, M., Letaw, J.H., Litman, G.W., Litman, R.T., Mikami, M., Ota, T., Saha, N.R., Williams, L., Stadler, P.F., Wang, H., Taylor, J.S., Fontenot, Q., Ferrara, A., Searle, S.M., Aken, B., Yandell, M., Schneider, I., Yoder, J.A., Volff, J.N., Meyer, A., Amemiya, C.T., Venkatesh, B., Holland, P.W., Guiguen, Y., Bobe, J., Shubin, N.H., Di Palma, F., Alföldi, J., Lindblad-Toh, K., Postlethwait, J.H. (2016) The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons. Nature Genetics. 48(4):427-37
Wang, Y., Li, S., Liu, W., Wang, F., Hu, L.F., Zhong, Z.M., Wang, H., Liu, C.F. (2016) Vesicular Monoamine Transporter 2 (Vmat2)knockdown elicits anxiety-like behavior in zebrafish. Biochemical and Biophysical Research Communications. 470(4):792-7
Sheng, L., Wang, L., Zhao, X., Hu, R., Yu, X., Hong, J., Liu, D., Xu, B., Zhu, Y., Wang, H., Hong, F. (2016) Mechanism of TiO2 nanoparticle-induced neurotoxicity in zebrafish (Danio rerio). Environmental toxicology. 31(2):163-75
Wang, F., Huang, G.D., Tian, H., Zhong, Y.B., Shi, H.J., Li, Z., Zhang, X.S., Wang, H., Sun, F. (2015) Point mutations in KAL1 and the mitochondrial gene MT-tRNA(cys) synergize to produce Kallmann syndrome phenotype. Scientific Reports. 5:13050
Ren, D.L., Li, Y.J., Hu, B.B., Wang, H., Hu, B. (2015) Melatonin regulates the rhythmic migration of neutrophils in live zebrafish. Journal of pineal research. 58(4):452-60
Wang, M., Zhong, Z., Zhong, Y., Zhang, W., Wang, H. (2015) The Zebrafish Period2 Protein Positively Regulates the Circadian Clock through Mediation of Retinoic Acid Receptor (RAR)-related Orphan Receptor α (Rorα). The Journal of biological chemistry. 290(7):4367-4382
Huang, J., Zhong, Z., Wang, M., Chen, X., Tan, Y., Zhang, S., He, W., He, X., Huang, G., Lu, H., Wu, P., Che, Y., Yan, Y.L., Postlethwait, J.H., Chen, W., Wang, H. (2015) Circadian modulation of dopamine levels and dopaminergic neuron development contributes to attention deficiency and hyperactive behavior. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35:2572-87
Liu, C., Hu, J., Qu, C., Wang, L., Huang, G., Niu, P., Zhong, Z., Hong, F., Wang, G., Postlethwait, J.H., Wang, H. (2015) Molecular evolution and functional divergence of zebrafish (Danio rerio) cryptochrome genes. Scientific Reports. 5:8113
Liu, S., Wang, M.X., Mao, C.J., Cheng, X.Y., Wang, C.T., Huang, J., Zhong, Z.M., Hu, W.D., Wang, F., Hu, L.F., Wang, H., Liu, C.F. (2014) Expression and functions of ASIC1 in the zebrafish retina. Biochemical and Biophysical Research Communications. 455(3-4):353-7
Zhang, S., Xu, M., Huang, J., Tang, L., Zhang, Y., Wu, J., Lin, S., and Wang, H. (2014) Heme acts through the Bach1b/Nrf2a-MafK pathway to regulate exocrine peptidase precursor genes in porphyric zebrafish. Disease models & mechanisms. 7(7):837-45
Chen, B., Yan, Y.L., Liu, C., Bo, L., Li, G.F., Wang, H., and Xu, Y.J. (2014) Therapeutic effect of deferoxamine on iron overload-induced inhibition of osteogenesis in a zebrafish model. Calcified tissue international. 94(3):353-360
Wang, H. (2009) Comparative genomic analysis of teleost fish bmal genes. Genetica. 136(1):149-161
Wang, H. (2008) Comparative Analysis of Period Genes in Teleost Fish Genomes. Journal of molecular evolution. 67(1):29-40
Wang, H. (2008) Comparative analysis of teleost fish genomes reveals preservation of different ancient clock duplicates in different fishes. Marine genomics. 1(2):69-78
Wang, H., Kesinger, J.W., Zhou, Q., Wren, J.D., Martin, G., Turner, S., Tang, Y., Frank, M.B., and Centola, M. (2008) Identification and characterization of zebrafish ocular formation genes. Genome. 51(3):222-235
Wang, H., Zhou, Q., Kesinger, J.W., Norris, C., and Valdez, C. (2007) Heme regulates exocrine peptidase precursor genes in zebrafish. Experimental Biology and Medicine. 232(9):1170-1180
Wang, H., Lee, E.M., Sperber, S.M., Lin, S., Ekker, M., and Long, Q. (2007) Isolation and expression of zebrafish zinc-finger transcription factor gene tsh1. Gene expression patterns : GEP. 7(3):318-322
Tucker, A.E., Salles, I.I., Voth, D.E., Ortiz-Leduc, W., Wang, H., Dozmorov, I., Centola, M., and Ballard, J.D. (2003) Decreased glycogen synthase kinase 3-beta levels and related physiological changes in Bacillus anthracis lethal toxin-treated macrophages. Cellular Microbiology. 5(8):523-532
Young, R.M., Marty, S., Nakano, Y., Wang, H., Yamamoto, D., Lin, S., and Allende, M.L. (2002) Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 223(2):298-305
Wang, H., Long, Q.M., Marty, S.D., Sassa, S., and Lin, S. (1998) A zebrafish model for hepatoerythropoietic porphyria. Nature Genetics. 20:239-243
Long, Q.M., Meng, A.M., Wang, H., Jessen, J.R., Farrell, M.J., and Lin, S. (1997) GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene. Development (Cambridge, England). 124(20):4105-4111

NON-ZEBRAFISH PUBLICATIONS
Wang, H., E. D. McArthur, and D. C. Freeman. 1999. Narrow hybrid zone between two subspecies of big sagebrush (Artemisia tridentata: Asteraceae). IX. Elemental uptake and niche differentiation. American Journal of Botany 86:1099-1107.

Freeman, D. C., H. Wang, S. C. Sanderson, and E. D. McArthur. 1999. Characterization of a narrow hybrid zone between two subspecies of big sagebrush (Artemisia tridentata: Asteraceae). VII. Demographic and community analysis. Evolutionary Ecology Research 1:487-502.

J. H. Graham, D. C. Freeman, H. Wang, and E. D. McArthur. 1999. Ecological analysis of the big sagebrush hybrid zone. In: McArthur, E. D.; Ostler, W. K.; Wambolt, C. L., comps. Proceedings: Shrub Ecotones, 1998 August 12-14, Ephraim, UT Proceedings RMRS-P-11, pages 11-14. Ogden, UT: U. S. Department of Agriculture, Forest Service, Rocky Mountain Research Station.

D. C. Freeman, K. J. Miglia, E. D. McArthur, J. H. Graham, and H. Wang.1999. Narrow Hybrid Zone Between Two Subspecies of Big Sagebrush (Artemisia tridentata: Asteraceae): X. Performance in Reciprocal Transplant Gardens. In: McArthur, E. D.; Ostler, W. K.; Wambolt, C. L., comps. Proceedings: Shrub Ecotones, 1998 August 12-14, Ephraim, UT Proceedings RMRS-P-11, pages 15-24. Ogden, UT: U. S. Department of Agriculture, Forest Service, Rocky Mountain Research Station.

Wang, H., D. W. Byrd, J. L. Howard, E. D. McArthur, J. H. Graham, and D. C. Freeman. 1998. Narrow hybrid zone between two subspecies of big sagebrush (Artemisia tridentata: Asteraceae). V. Soil properties. International Journal of Plant Sciences 159:139-147.

McArthur, E. D., D. C. Freeman, J. H. Graham, H. Wang, S. C. Sanderson, and B. N. Smith. 1998. Narrow hybrid zone between two subspecies of big sagebrush (Artemisia tridentata: Asteraceae). VI. Respiration and water potential. Canadian Journal of Botany 76:567-574.

Byrd, D. W., E. D. McArthur, H. Wang, and D. C. Freeman. 1998. Narrow hybrid zone between two subspecies of big sagebrush (Artemisia tridentata: Asteraceae). VIII. Temporal and spatial patterns of terpenes. Biochemical Systematics and Ecology 27:11-25.

Wang, H., E. D. McArthur, S. C. Sanderson, J. H. Graham, and D. C. Freeman. 1997. Narrow hybrid zone between two subspecies of big sagebrush (Artemisia tridentata: Asteraceae). IV. Reciprocal transplant experiments. Evolution 51:95-102.