PUBLICATION

Live analysis of endodermal layer formation identifies random walk as a novel gastrulation movement

Authors
Pézeron, G., Mourrain, P., Courty, S., Ghislain, J., Becker, T.S., Rosa, F.M., and David, N.B.
ID
ZDB-PUB-080309-18
Date
2008
Source
Current biology : CB   18(4): 276-281 (Journal)
Registered Authors
Becker, Thomas S., David, Nicholas, Mourrain, Philippe, Pézeron, Guillaume, Rosa, Frederic
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/physiology
  • Cell Movement/physiology*
  • Embryonic Induction/physiology
  • Endoderm/cytology*
  • Endoderm/physiology
  • Gastrulation/physiology*
  • High Mobility Group Proteins/metabolism
  • Nodal Protein
  • SOX Transcription Factors
  • Transcription Factors/metabolism
  • Transforming Growth Factor beta/metabolism
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Zebrafish Proteins/metabolism
PubMed
18291651 Full text @ Curr. Biol.
Abstract
During gastrulation, dramatic movements rearrange cells into three germ layers expanded over the entire embryo [1-3]. In fish, both endoderm and mesoderm are specified as a belt at the embryo margin. Mesodermal layer expansion is achieved through the combination of two directed migrations. The outer ring of precursors moves toward the vegetal pole and continuously seeds mesodermal cells inside the embryo, which then reverse their movement in the direction of the animal pole [3-6]. Unlike mesoderm, endodermal cells internalize at once and must therefore adopt a different strategy to expand over the embryo [7, 8]. With live imaging of YFP-expressing zebrafish endodermal cells, we demonstrate that in contrast to mesoderm, internalized endodermal cells display a nonoriented/noncoordinated movement fit by a random walk that rapidly disperses them over the yolk surface. Transplantation experiments reveal that this behaviour is largely cell autonomous, induced by TGF-beta/Nodal, and dependent on the downstream effector Casanova. At midgastrulation, endodermal cells switch to a convergence movement. We demonstrate that this switch is triggered by environmental cues. These results uncover random walk as a novel Nodal-induced gastrulation movement and as an efficient strategy to transform a localized cell group into a layer expanded over the embryo.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes