ZFIN ID: ZDB-PUB-080228-10
Tjp3/zo-3 is critical for epidermal barrier function in zebrafish embryos
Kiener, T.K., Selptsova-Friedrich, I., and Hunziker, W.
Date: 2008
Source: Developmental Biology   316(1): 36-49 (Journal)
Registered Authors:
Keywords: Tight junction, Zonula occludens, Enveloping cell layer, Epidermis, Kidney, Diffusion barrier, Osmoregulation
MeSH Terms:
  • Animals
  • Cell Membrane Permeability/drug effects
  • Cell Membrane Permeability/genetics
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/drug effects
  • Embryonic Development/genetics
  • Epidermis/drug effects
  • Epidermis/embryology*
  • Epidermis/metabolism*
  • Membrane Proteins/antagonists & inhibitors
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Membrane Proteins/physiology*
  • Oligonucleotides, Antisense/pharmacology
  • Osmotic Pressure/drug effects
  • Tight Junctions/metabolism
  • Tight Junctions/ultrastructure
  • Water-Electrolyte Balance*/drug effects
  • Water-Electrolyte Balance*/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Zonula Occludens Proteins
PubMed: 18275946 Full text @ Dev. Biol.
FIGURES
ABSTRACT
TJP3/ZO-3 is a scaffolding protein that tethers tight junction integral membrane proteins to the actin cytoskeleton and links the conserved Crumbs polarity complex to tight junctions. The physiological function of TJP3/ZO-3 is not known and mice lacking TJP3/ZO-3 show no apparent phenotype. Here we show that Tjp3/Zo-3 is a component of tight junctions present in the enveloping cell layer of zebrafish embryos. Silencing tjp3/zo-3 using morpholinos leads to edema, loss of blood circulation and tail fin malformations in the embryos. The ultrastructure of tight junctions of the enveloping cell layer is disrupted, without affecting the asymmetric distribution of plasma membrane proteins. Morphants show a loss of the epidermal barrier, as assessed by an increased permeability of the enveloping cell layer to low molecular weight tracers and a higher sensitivity of the embryos to osmotic stress. Subjecting wild-type embryos to osmotic stress mimicks the morphant phenotype, consistent with the phenotype being a direct consequence of failed osmoregulation. Thus, Tjp3/Zo-3 is critical for barrier function of the enveloping cell layer and osmoregulation in early stages of zebrafish development.
ADDITIONAL INFORMATION