PUBLICATION

MyRIP anchors protein kinase A to the exocyst complex

Authors
Goehring, A.S., Pedroja, B.S., Hinke, S.A., Langeberg, L.K., and Scott, J.D.
ID
ZDB-PUB-070912-9
Date
2007
Source
The Journal of biological chemistry   282(45): 33155-33167 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • A Kinase Anchor Proteins/chemistry
  • A Kinase Anchor Proteins/genetics
  • A Kinase Anchor Proteins/metabolism
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Cell Line
  • Cyclic AMP-Dependent Protein Kinases/classification
  • Cyclic AMP-Dependent Protein Kinases/genetics
  • Cyclic AMP-Dependent Protein Kinases/metabolism*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Protein Binding
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Vesicular Transport Proteins/chemistry
  • Vesicular Transport Proteins/genetics
  • Vesicular Transport Proteins/metabolism*
  • Zebrafish
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 23)
PubMed
17827149 Full text @ J. Biol. Chem.
Abstract
The movement of signal transduction enzymes in and out of multi-protein complexes coordinates the spatial and temporal resolution of cellular events. Anchoring and scaffolding proteins are key to this process as they sequester protein kinases and phosphatases with a subset of their preferred substrates. The A-kinase anchoring family of proteins (AKAPs), which target the cAMP-dependent protein kinase (PKA) and other enzymes to defined subcellular microenvironments, represent a well-studied group of these signal-organizing molecules. In this report we demonstrate that the Rab27a GTPase effector protein MyRIP is a member of the AKAP family. The zebrafish homolog of MyRIP (Ze-AKAP2) was initially detected in a two-hybrid screen for AKAPs. A combination of biochemical, cell-based and immunofluorescence approaches demonstrate that the mouse MyRIP ortholog targets the type II PKA holoenzyme via an atypical mechanism to a specific perinuclear region of insulin-secreting cells. Similar approaches show that MyRIP interacts with the Sec6 and Sec8 components of the exocyst complex, an evolutionarily conserved protein unit that controls protein trafficking and exocytosis. These data indicate that MyRIP functions as a scaffolding protein that links PKA to components of the exocytosis machinery.
Genes / Markers
Marker Marker Type Name
myripbGENEmyosin VIIA and Rab interacting protein b
prkar2aaGENEprotein kinase, cAMP-dependent, regulatory, type II, alpha A
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Figures
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Expression
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Phenotype
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Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Fish
No data available
Antibodies
No data available
Orthology
Gene Orthology
myripb
Species Symbol Chromosome Accession # Evidence
HumanMYRIP3
Phylogenetic tree (1)
Conserved genome location (synteny) (1)
Amino acid sequence comparison (1)
MouseMyrip9
Amino acid sequence comparison (1)
Conserved genome location (synteny) (1)
Phylogenetic tree (1)
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Engineered Foreign Genes
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Mapping
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