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ZIRC
ZFIN ID: ZDB-PUB-070427-10
A major zebrafish polymorphism resource for genetic mapping
Bradley, K.M., Elmore, J.B., Breyer, J.P., Yaspan, B.L., Jessen, J.R., Knapik, E.W., and Smith, J.R.
Date: 2007
Source: Genome biology 8(4): R55 (Journal)
Registered Authors: Jessen, Jason R., Knapik, Ela W.
Keywords: none
MeSH Terms:
  • Animals
  • Chromosome Mapping/methods*
  • Cloning, Molecular
  • Genetic Linkage
  • Genetic Markers
  • Polymorphism, Single Nucleotide*
  • Zebrafish/genetics*
PubMed: 17428331 Full text @ Genome Biol.
ABSTRACT
We identify 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% - 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We position half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.
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