ZFIN ID: ZDB-PUB-070427-10
A major zebrafish polymorphism resource for genetic mapping
Bradley, K.M., Elmore, J.B., Breyer, J.P., Yaspan, B.L., Jessen, J.R., Knapik, E.W., and Smith, J.R.
We identify 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% - 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We position half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.