PUBLICATION
In vivo time-lapse imaging shows dynamic oligodendrocyte progenitor behavior during zebrafish development
- Authors
- Kirby, B.B., Takada, N., Latimer, A.J., Shin, J., Carney, T.J., Kelsh, R.N., and Appel, B.
- ID
- ZDB-PUB-061116-14
- Date
- 2006
- Source
- Nature Neuroscience 9(12): 1506-1511 (Journal)
- Registered Authors
- Appel, Bruce, Carney, Tom, Kelsh, Robert, Kirby, Brandon, Latimer, Andrew, Shin, Jimann, Takada, Norio
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Differentiation/physiology*
- Cell Lineage/physiology
- Cell Movement/physiology*
- Microscopy, Fluorescence
- Myelin Sheath/physiology
- Oligodendroglia/cytology*
- Spinal Cord/cytology*
- Spinal Cord/growth & development
- Stem Cells/cytology*
- Zebrafish
- PubMed
- 17099706 Full text @ Nat. Neurosci.
Citation
Kirby, B.B., Takada, N., Latimer, A.J., Shin, J., Carney, T.J., Kelsh, R.N., and Appel, B. (2006) In vivo time-lapse imaging shows dynamic oligodendrocyte progenitor behavior during zebrafish development. Nature Neuroscience. 9(12):1506-1511.
Abstract
Myelinating oligodendrocytes arise from migratory and proliferative oligodendrocyte progenitor cells (OPCs). Complete myelination requires that oligodendrocytes be uniformly distributed and form numerous, periodically spaced membrane sheaths along the entire length of target axons. Mechanisms that determine spacing of oligodendrocytes and their myelinating processes are not known. Using in vivo time-lapse confocal microscopy, we show that zebrafish OPCs continuously extend and retract numerous filopodium-like processes as they migrate and settle into their final positions. Process remodeling and migration paths are highly variable and seem to be influenced by contact with neighboring OPCs. After laser ablation of oligodendrocyte-lineage cells, nearby OPCs divide more frequently, orient processes toward the ablated cells and migrate to fill the unoccupied space. Thus, process activity before axon wrapping might serve as a surveillance mechanism by which OPCs determine the presence or absence of nearby oligodendrocyte-lineage cells, facilitating uniform spacing of oligodendrocytes and complete myelination.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping