PUBLICATION
High-throughput zebrafish histology
- Authors
- Sabaliauskas, N.A., Foutz, C.A., Mest, J.R., Budgeon, L.R., Sidor, AT, Gershenson, J.A., Joshi, S.B., and Cheng, K.C.
- ID
- ZDB-PUB-060731-14
- Date
- 2006
- Source
- Methods (San Diego, Calif.) 39(3): 246-254 (Journal)
- Registered Authors
- Cheng, Keith C., Mest, Jason, Sabaliauskas, Nicole
- Keywords
- Zebrafish, Histology, High-throughput toxicology method, Atlas screen, Array genetic screen
- MeSH Terms
-
- Animals
- Histological Techniques*/instrumentation
- Larva
- Microtomy/instrumentation
- Paraffin Embedding/instrumentation
- Phenotype
- Software
- Tissue Array Analysis/methods*
- Tissue Fixation
- Zebrafish/anatomy & histology*
- Zebrafish/growth & development
- PubMed
- 16870470 Full text @ Methods
Citation
Sabaliauskas, N.A., Foutz, C.A., Mest, J.R., Budgeon, L.R., Sidor, AT, Gershenson, J.A., Joshi, S.B., and Cheng, K.C. (2006) High-throughput zebrafish histology. Methods (San Diego, Calif.). 39(3):246-254.
Abstract
The morphological effects of mutation and disease are often critical to our understanding of normal and abnormal function. The power and popularity of zebrafish as a forward and reverse genetic vertebrate model system, combined with its small size, have made it an ideal model in which to study the genetics of histologically scorable phenotypes. The presence of multiple tissue types in this organism's small larvae also makes it a potentially important model for toxicological analysis. Studying histological phenotypes is greatly enhanced by high-throughput methods of histology. Here, we describe details of high-throughput histology of the zebrafish using larval arrays, along with recent advances in mold design and discussion of work in progress that will lead to easier ways for people in the field to more rapidly score phenotypes in arrays. These detailed descriptions, together with the troubleshooting guide, should enable any laboratory with ties to a histology facility to perform high-throughput histology of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping