ZFIN ID: ZDB-PUB-060508-13
BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis
Gupta, S., Zhu, H., Zon, L.I., and Evans, T.
Date: 2006
Source: Development (Cambridge, England)   133(11): 2177-2187 (Journal)
Registered Authors: Evans, Todd, Zon, Leonard I.
Keywords: Hematopoiesis, Endothelial, Pronephros, Transgenic, Zebrafish, lmo2
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Biomarkers
  • Bone Morphogenetic Protein Receptors/genetics
  • Bone Morphogenetic Protein Receptors/metabolism
  • Bone Morphogenetic Proteins/metabolism*
  • Deoxyribonucleases, Type II Site-Specific/genetics
  • Deoxyribonucleases, Type II Site-Specific/metabolism
  • Gene Expression Regulation, Developmental
  • Hematopoiesis*
  • Kidney/blood supply
  • Kidney/embryology
  • Kidney/metabolism
  • Mesoderm/metabolism*
  • PAX2 Transcription Factor/metabolism
  • Saccharomyces cerevisiae Proteins
  • Signal Transduction*
  • Somites/metabolism*
  • Stem Cells/metabolism
  • Time Factors
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
PubMed: 16672337 Full text @ Development
The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.