ZFIN ID: ZDB-PUB-060508-13
BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis
Gupta, S., Zhu, H., Zon, L.I., and Evans, T.
Date: 2006
Source: Development (Cambridge, England) 133(11): 2177-2187 (Journal)
Registered Authors: Evans, Todd, Zon, Leonard I.
Keywords: Hematopoiesis, Endothelial, Pronephros, Transgenic, Zebrafish, lmo2
MeSH Terms: Animals; Animals, Genetically Modified; Biomarkers; Bone Morphogenetic Protein Receptors/genetics; Bone Morphogenetic Protein Receptors/metabolism (all 24) expand
PubMed: 16672337 Full text @ Development
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ABSTRACT
The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.
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