Animals; Animals, Genetically Modified; Biomarkers; Bone Morphogenetic Protein Receptors/genetics; Bone Morphogenetic Protein Receptors/metabolism
Animals; Animals, Genetically Modified; Biomarkers; Bone Morphogenetic Protein Receptors/genetics; Bone Morphogenetic Protein Receptors/metabolism; Bone Morphogenetic Proteins/metabolism*; Deoxyribonucleases, Type II Site-Specific/genetics; Deoxyribonucleases, Type II Site-Specific/metabolism; Gene Expression Regulation, Developmental; Hematopoiesis*; Kidney/blood supply; Kidney/embryology; Kidney/metabolism; Mesoderm/metabolism*; PAX2 Transcription Factor/metabolism; Saccharomyces cerevisiae Proteins; Signal Transduction*; Somites/metabolism*; Stem Cells/metabolism; Time Factors; Zebrafish/embryology; Zebrafish/genetics; Zebrafish/metabolism; Zebrafish Proteins/metabolism
The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.