ZFIN ID: ZDB-PUB-050518-20
Transgenic zebrafish that express tyrosine hydroxylase promoter in inner retinal cells
Gao, Y., Li, P., and Li, L.
Date: 2005
Source: Developmental dynamics : an official publication of the American Association of Anatomists   233(3): 921-929 (Journal)
Registered Authors: Li, Lei
Keywords: catecholamine; transgenic; retina; development; zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Brain/embryology
  • Brain/enzymology
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter/genetics
  • Phenylethanolamine N-Methyltransferase/genetics
  • Phenylethanolamine N-Methyltransferase/metabolism
  • Promoter Regions, Genetic/genetics*
  • Rats
  • Retina/cytology*
  • Retina/embryology
  • Retina/enzymology
  • Retina/metabolism*
  • Transgenes/genetics
  • Tyrosine 3-Monooxygenase/genetics*
  • Tyrosine 3-Monooxygenase/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • gamma-Aminobutyric Acid/metabolism
PubMed: 15895407 Full text @ Dev. Dyn.
We have generated a transgenic zebrafish line [Tg(Th:GFP)] that expresses green fluorescence proteins (GFP) driven by rat tyrosine hydroxylase (TH) promoter. In zebrafish, the transgene was expressed as early as 16 hr postfertilization (hpf). The first transgene expression was detected in the midbrain. Within a few hours of development, the expression spread to the forebrain and hindbrain. In the retina, the first transgene expression was detected at approximately 40 hpf, at which time a single GFP-positive cell was seen in the ventral-nasal patch of the retina. In late development, GFP spread across the inner retina. GFP was found in retinal cells that expressed TH or phenylethanolamine N-methyl-transferase (PNMT), the first and last enzymes for synthesis of catecholamine, respectively. This suggests that the transgene is expressed in catecholaminergic neurons. Of interest, GFP was also detected in some retinal cells that release gamma-aminobutyric acid. These latter data suggest that the transgene may also be expressed in noncatecholaminergic cells. Developmental Dynamics, 2005. (c) 2005 Wiley-Liss, Inc.