Kudoh, T., Tsang, M., Hukriede, N.A., Chen, X., Dedekian, M., Clarke, C.J., Kiang, A., Schultz, S., Epstein, J.A., Toyama, R., and Dawid, I.B. (2001) A gene expression screen in zebrafish embryogenesis. ZFIN Direct Data Submission. . (http://zfin.org).
A screen for developmentally regulated genes was conducted in the zebrafish, a system offering substantial advantages
for the study of the molecular genetics of vertebrate embryogenesis. Clones from a normalized cDNA library from early
somitogenesis stages were picked randomly and tested by high-throughput in situ hybridization for restricted expression
in at least one of four stages of development. Among 2765 clones that were screened, a total of 347 genes with
patterns judged to be restricted were selected. These clones were subjected to partial sequence analysis, allowing
recognition of functional motifs in 163 among them. In addition, a portion of the clones were mapped with the aid of the
LN54 radiation hybrid panel. The usefulness of the in situ hybridization screening approach is illustrated by describing
several new markers for the characteristic structure in the fish embryo named the yolk syncytial layer, and for different
regions of the developing brain.
Acknowledging this work:
All clones, information, and images used from this work should cite the published description of this screen:
Genome Res. 11(12):1979-1987