ZFIN ID: ZDB-PUB-050201-3
Two ribeye Genes in Teleosts: The Role of Ribeye in Ribbon Formation and Bipolar Cell Development
Wan, L., Almers, W., and Chen, W.
Date: 2005
Source: The Journal of neuroscience : the official journal of the Society for Neuroscience 25(4): 941-949 (Journal)
Registered Authors: Chen, Wenbiao
Keywords: Ribeye; optokinetic response; retina; synaptic ribbon; synaptogenesis; bipolar cells; apoptosis; morpholino antisense oligo
MeSH Terms: Animals; Apoptosis/physiology; Eye Proteins/genetics; Eye Proteins/physiology*; Gene Expression Regulation, Developmental (all 21) expand
PubMed: 15673675 Full text @ J. Neurosci.
FIGURES   (current status)
Ribeye is the only known protein specific to synaptic ribbon, but its function is unclear. We show that the teleost fish, Fugu and zebrafish, have two ribeye genes, ribeye a and ribeye b. Whole-mount in situ hybridization revealed that ribeye a is expressed in tissues containing synaptic ribbons, including the pineal gland, inner ear, and retina. Ribeye b is absent in the pineal gland. In the retina, ribeye a is expressed in both photoreceptors and bipolar cells, whereas ribeye b is detected only in photoreceptors. To study the function of Ribeye a in retina, we depleted it by morpholino antisense oligos. Fish deficient in Ribeye a lack an optokinetic response and have shorter synaptic ribbons in photoreceptors and fewer synaptic ribbons in bipolar cells. Their bipolar cells still target Syntaxin-3 proteins to the inner plexiform layer and have abundant vsx1 mRNA. However, they lack large synaptic terminals and show increased apoptosis. Rod bipolar cells are fewer in number and/or deficient in PKCalpha. Recovery of Ribeye a levels rescues the optokinetic response, increases the number of PKCalpha-positive bipolar cells, and stops apoptosis. We conclude that Ribeye a is important for late steps in bipolar cell development.