ZFIN ID: ZDB-PUB-041022-2
Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos
Herbomel, P., and Levraud, J.P.
Date: 2005
Source: Methods in molecular medicine   105: 199-214 (Chapter)
Registered Authors: Herbomel, Philippe, Levraud, Jean-Pierre
Keywords:  Zebrafish; macrophages; DIC microscopy; Nomarski; video microscopy; embryo; hematopoiesis
MeSH Terms:
  • Animals
  • Cell Differentiation/physiology*
  • Cell Movement/physiology*
  • Macrophages/physiology*
  • Microscopy, Confocal/methods*
  • Microscopy, Video/methods*
  • Zebrafish/embryology*
PubMed: 15492397
Because zebrafish embryos are transparent, cell behaviors and interactions can be directly imaged noninvasely in live embryos using differential interference contrast-Nomarski light microscopy. We found that the imaging quality can be much improved by coupling differential interference contrast-Nomarski to true (analogical) color video so as to visualize the image in real time on a high-resolution colour video monitor. We explain here how to apply this approach to the in vivo imaging of embryonic macrophages, which constitute a distinct early macrophage lineage, that originates from the rostral-most lateral mesoderm -adjacent to the cardiac field, differentiate in the yolk sac, and rapidly spread in embryonic tissues, although still retaining proliferative capacity.