|ZFIN ID: ZDB-PUB-041022-2|
Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos
Herbomel, P., and Levraud, J.P.
|Source:||Methods in molecular medicine 105: 199-214 (Chapter)|
|Registered Authors:||Herbomel, Philippe, Levraud, Jean-Pierre|
|Keywords:||Zebrafish; macrophages; DIC microscopy; Nomarski; video microscopy; embryo; hematopoiesis|
Herbomel, P., and Levraud, J.P. (2005) Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos. Methods in molecular medicine. 105:199-214.
ABSTRACTBecause zebrafish embryos are transparent, cell behaviors and interactions can be directly imaged noninvasely in live embryos using differential interference contrast-Nomarski light microscopy. We found that the imaging quality can be much improved by coupling differential interference contrast-Nomarski to true (analogical) color video so as to visualize the image in real time on a high-resolution colour video monitor. We explain here how to apply this approach to the in vivo imaging of embryonic macrophages, which constitute a distinct early macrophage lineage, that originates from the rostral-most lateral mesoderm -adjacent to the cardiac field, differentiate in the yolk sac, and rapidly spread in embryonic tissues, although still retaining proliferative capacity.
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