PUBLICATION

Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos

Authors
Herbomel, P., and Levraud, J.P.
ID
ZDB-PUB-041022-2
Date
2005
Source
Methods in molecular medicine   105: 199-214 (Chapter)
Registered Authors
Herbomel, Philippe, Levraud, Jean-Pierre
Keywords
 Zebrafish; macrophages; DIC microscopy; Nomarski; video microscopy; embryo; hematopoiesis
MeSH Terms
  • Animals
  • Cell Differentiation/physiology*
  • Cell Movement/physiology*
  • Macrophages/physiology*
  • Microscopy, Confocal/methods*
  • Microscopy, Video/methods*
  • Zebrafish/embryology*
PubMed
15492397 Full text @ Methods Mol. Med.
Abstract
Because zebrafish embryos are transparent, cell behaviors and interactions can be directly imaged noninvasely in live embryos using differential interference contrast-Nomarski light microscopy. We found that the imaging quality can be much improved by coupling differential interference contrast-Nomarski to true (analogical) color video so as to visualize the image in real time on a high-resolution colour video monitor. We explain here how to apply this approach to the in vivo imaging of embryonic macrophages, which constitute a distinct early macrophage lineage, that originates from the rostral-most lateral mesoderm -adjacent to the cardiac field, differentiate in the yolk sac, and rapidly spread in embryonic tissues, although still retaining proliferative capacity.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes