PUBLICATION
The zebrafish onecut gene hnf-6 functions in an evolutionarily conserved genetic pathway that regulates vertebrate biliary development
- Authors
- Matthews, R.P., Lorent, K., Russo, P., and Pack, M.
- ID
- ZDB-PUB-041004-5
- Date
- 2004
- Source
- Developmental Biology 274(2): 245-259 (Journal)
- Registered Authors
- Lorent, Kristin, Matthews, Randy, Pack, Michael
- Keywords
- Biliary development; Zebrafish; hnf-6
- MeSH Terms
-
- Biological Evolution*
- Lipids
- In Situ Hybridization
- Trans-Activators/genetics*
- Trans-Activators/metabolism*
- Animals
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Base Sequence
- Bile Ducts/embryology
- Bile Ducts/ultrastructure
- Humans
- Molecular Sequence Data
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/metabolism*
- Pancreas/cytology
- Pancreas/embryology
- Pancreas/metabolism
- Sequence Alignment
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- RNA, Messenger/metabolism
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology
- Gene Expression Regulation, Developmental
- Zebrafish Proteins
- Liver/embryology*
- Liver/metabolism
- Liver/physiology
- Liver/ultrastructure
- Hepatocyte Nuclear Factor 6
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Amino Acid Sequence
- Hepatocyte Nuclear Factor 1-beta
- PubMed
- 15385156 Full text @ Dev. Biol.
Citation
Matthews, R.P., Lorent, K., Russo, P., and Pack, M. (2004) The zebrafish onecut gene hnf-6 functions in an evolutionarily conserved genetic pathway that regulates vertebrate biliary development. Developmental Biology. 274(2):245-259.
Abstract
Targeted disruption of the onecut transcription factor, hnf-6, alters mammalian biliary system development. We have identified a related zebrafish cDNA expressed in the developing liver that is a functional ortholog of mammalian hnf-6. Antisense-mediated knockdown of zebrafish hnf-6 perturbs development of the intrahepatic biliary system. Knockdown of zebrafish hnf-6 alters expression of vhnf1 and the zebrafish orthologs of other mammalian genes regulated by hnf-6. Coinjection of mRNA encoding zebrafish vhnf1 rescues the biliary phenotype of hnf-6 morphants. These experiments strongly suggest that hnf-6 and vhnf1 function within an evolutionarily conserved pathway that regulates biliary development. Forced expression of either hnf-6 or vhnf1 also produces biliary phenotypes. Altered bile duct development in both loss- and gain-of-function experiments suggests that zebrafish biliary cells are sensitive to the dosage of hnf-6-mediated gene transcription.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping