ZFIN ID: ZDB-PUB-040706-7
Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1
Poss, K.D., Nechiporuk, A., Stringer, K.F., Lee, C., and Keating, M.T.
Date: 2004
Source: Genes and Development 18(13): 1527-1532 (Journal)
Registered Authors: Keating, Mark T., Lee, Charles, Nechiporuk, Alex, Poss, Kenneth D.
Keywords: Zebrafish, aneuploidy, meiosis, spindle checkpoint, Mps1, nondisjunction
MeSH Terms: Aneuploidy*; Animals; Cell Cycle Proteins*; Chromosome Aberrations; Embryo, Nonmammalian/pathology (all 22) expand
PubMed: 15231734 Full text @ Genes Dev.
Aneuploidy, resulting from chromosome missegregation during meiosis, is a major cause of human infertility and birth defects. However, its molecular basis remains incompletely understood. Here we have identified a spectrum of chromosome anomalies in embryos of zebrafish homozygous for a hypomorphic mutation in Mps1, a kinase required for the mitotic checkpoint. These aneuploidies are caused by meiotic error and result in severe developmental defects. Our results reveal Mps1 as a critical regulator of chromosome number in zebrafish, and demonstrate how slight genetic perturbation of a mitotic checkpoint factor can dramatically reduce the fidelity of chromosome segregation during vertebrate meiosis.