ZFIN ID: ZDB-PUB-040706-7
Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1
Poss, K.D., Nechiporuk, A., Stringer, K.F., Lee, C., and Keating, M.T.
Date: 2004
Source: Genes and Development 18(13): 1527-1532 (Journal)
Registered Authors: Keating, Mark T., Lee, Charles, Nechiporuk, Alex, Poss, Kenneth D.
Keywords: Zebrafish, aneuploidy, meiosis, spindle checkpoint, Mps1, nondisjunction
MeSH Terms:
  • Aneuploidy*
  • Animals
  • Cell Cycle Proteins*
  • Chromosome Aberrations
  • Embryo, Nonmammalian/pathology
  • Female
  • Fetal Death/genetics
  • Male
  • Mitosis
  • Mutation
  • Oocytes/physiology*
  • Protein Kinases*
  • Protein-Serine-Threonine Kinases/genetics*
  • Protein-Serine-Threonine Kinases/metabolism
  • Protein-Tyrosine Kinases/genetics*
  • Protein-Tyrosine Kinases/metabolism
  • Spermatozoa/physiology*
  • Testis/physiology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 15231734 Full text @ Genes Dev.
ABSTRACT
Aneuploidy, resulting from chromosome missegregation during meiosis, is a major cause of human infertility and birth defects. However, its molecular basis remains incompletely understood. Here we have identified a spectrum of chromosome anomalies in embryos of zebrafish homozygous for a hypomorphic mutation in Mps1, a kinase required for the mitotic checkpoint. These aneuploidies are caused by meiotic error and result in severe developmental defects. Our results reveal Mps1 as a critical regulator of chromosome number in zebrafish, and demonstrate how slight genetic perturbation of a mitotic checkpoint factor can dramatically reduce the fidelity of chromosome segregation during vertebrate meiosis.
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