PUBLICATION
Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1
- Authors
- Poss, K.D., Nechiporuk, A., Stringer, K.F., Lee, C., and Keating, M.T.
- ID
- ZDB-PUB-040706-7
- Date
- 2004
- Source
- Genes & Development 18(13): 1527-1532 (Journal)
- Registered Authors
- Keating, Mark T., Lee, Charles, Nechiporuk, Alex, Poss, Kenneth D.
- Keywords
- Zebrafish, aneuploidy, meiosis, spindle checkpoint, Mps1, nondisjunction
- MeSH Terms
-
- Protein-Tyrosine Kinases/genetics*
- Protein-Tyrosine Kinases/metabolism
- Protein Serine-Threonine Kinases/genetics*
- Protein Serine-Threonine Kinases/metabolism
- Embryo, Nonmammalian/pathology
- Aneuploidy*
- Spermatozoa/physiology*
- Mitosis
- Mutation
- Fetal Death/genetics
- Protein Kinases*
- Female
- Male
- Zebrafish/embryology
- Zebrafish/genetics
- Testis/physiology
- Animals
- Oocytes/physiology*
- Chromosome Aberrations
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Cell Cycle Proteins*
- PubMed
- 15231734 Full text @ Genes & Dev.
Citation
Poss, K.D., Nechiporuk, A., Stringer, K.F., Lee, C., and Keating, M.T. (2004) Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1. Genes & Development. 18(13):1527-1532.
Abstract
Aneuploidy, resulting from chromosome missegregation during meiosis, is a major cause of human infertility and birth defects. However, its molecular basis remains incompletely understood. Here we have identified a spectrum of chromosome anomalies in embryos of zebrafish homozygous for a hypomorphic mutation in Mps1, a kinase required for the mitotic checkpoint. These aneuploidies are caused by meiotic error and result in severe developmental defects. Our results reveal Mps1 as a critical regulator of chromosome number in zebrafish, and demonstrate how slight genetic perturbation of a mitotic checkpoint factor can dramatically reduce the fidelity of chromosome segregation during vertebrate meiosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping