ZFIN ID: ZDB-PUB-040402-3
Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary
Geling, A., Plessy, C., Rastegar, S., Strähle, U., and Bally-Cuif, L.
Date: 2004
Source: Development (Cambridge, England) 131(9): 1993-2006 (Journal)
Registered Authors: Bally-Cuif, Laure, Geling, Andrea, Plessy, Charles, Rastegar, Sepand, Strähle, Uwe
Keywords: Her5, Hairy, Midbrain-hindbrain boundary, Zebrafish, Neurogenesis, Pre-patterning
MeSH Terms: Animals; Animals, Genetically Modified; Basic Helix-Loop-Helix Transcription Factors; Body Patterning*; Cell Differentiation/physiology (all 29) expand
PubMed: 15056616 Full text @ Development
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ABSTRACT
Neurogenesis in both vertebrates and invertebrates is tightly controlled in time and space involving both positive and negative regulators. We report here that the bHLH factor Her5 acts as a prepattern gene to prevent neurogenesis in the anlage of the midbrain/hindbrain boundary in the zebrafish neural plate. This involves selective suppression of both neurogenin1 (ngn1) and coe2 mRNA expression in a process that is independent of Notch signalling, and where inhibition of either ngn1 or coe2 expression is sufficient to prevent neuronal differentiation across the midbrain-hindbrain boundary. A ngn1 transgene faithfully responds to Her5 and deletion analysis of the transgene identifies an E-box in a ngn1 upstream enhancer to be required for repression by Her5. Together our data demonstrate a role of Her5 as a prepattern factor in the spatial definition of proneural domains in the zebrafish neural plate, in a manner similar to its Drosophila homologue Hairy.
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