PUBLICATION
Formation of regulator/target gene relationships during evolution
- Authors
- Schlake, T., Schorpp, M., and Boehm, T.
- ID
- ZDB-PUB-040205-7
- Date
- 2000
- Source
- Gene 256(1-2): 29-34 (Journal)
- Registered Authors
- Boehm, Tom, Schorpp, Michael
- Keywords
- Evolution; cis-Regulatory region; Hair follicle; Protein function; Transcription factor; Whn/Foxn1
- MeSH Terms
-
- Molecular Sequence Data
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Amino Acid Sequence
- Chordata, Nonvertebrate/genetics
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/physiology
- Gene Expression Regulation
- Sequence Analysis, DNA
- DNA/chemistry
- DNA/genetics
- Sequence Homology, Amino Acid
- Gene Expression
- Evolution, Molecular*
- Sequence Alignment
- Genes, Regulator/genetics*
- Genes, Regulator/physiology
- Animals
- Forkhead Transcription Factors
- Transcription Factors/genetics
- Transcription Factors/physiology
- Transcriptional Activation
- Drosophila melanogaster/genetics
- Zebrafish/genetics
- PubMed
- 11054532 Full text @ Gene
Citation
Schlake, T., Schorpp, M., and Boehm, T. (2000) Formation of regulator/target gene relationships during evolution. Gene. 256(1-2):29-34.
Abstract
The Foxn1-like forkhead/winged-helix transcription factor genes have been maintained in single copy throughout chordate evolution. Among other functions, Foxn1 (formerly known as Whn) regulates the expression of hair keratin genes in the hair follicle, which represents an evolutionarily novel organ characteristic of mammals. We show here that fish and mouse Foxn1-like genes are functionally equivalent in hair keratin gene activation, suggesting the absence of functionally relevant changes over the course of several hundred million years of vertebrate evolution. In contrast, the Foxn1-like gene from the cephalochordate Branchiostoma lanceolatum is inactive in this assay because of changes in the region located N-terminal to DNA binding and transcriptional activation domains of the protein. Our results indicate that functionally relevant changes in cis-regulatory regions are not necessarily accompanied by corresponding changes in transcription factor proteins in the formation of evolutionarily novel regulator/target gene relationships.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping