ZFIN ID: ZDB-PUB-031001-4
Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase alpha1 subunit gene (atp1a1a.5)
Blasiole, B., Canfield, V., Degrave, A., Thisse, C., Thisse, B., Rajarao, J., and Levenson, R.
Date: 2002
Source: Mechanisms of Development   (Suppl.) 119: S211-S214 (Journal)
Registered Authors: Blasiole, Brian, Canfield, Victor, Degrave, Agnes, Levenson, Robert, Rajarao, S. Johannes, Thisse, Bernard, Thisse, Christine
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • In Situ Hybridization
  • Molecular Sequence Data
  • Sodium-Potassium-Exchanging ATPase*
  • Zebrafish*/genetics
PubMed: 14516687 Full text @ Mech. Dev.
We have identified and characterized cDNAs encoding a novel zebrafish Na,K-ATPase alpha subunit. The full-length cDNA encodes a 1023-amino-acid-long peptide which shows greatest homology to zebrafish alpha1 polypeptides. Radiation hybrid mapping localized the new gene (atp1a1a.5) to linkage group 1 in close proximity to the previously identified cluster of Na,K-ATPase alpha1 genes. The expression of atp1a1a.5 in zebrafish embryos was analyzed using whole-mount in situ hybridization. From mid-somitogenesis through 48 h post fertilization (hpf), atp1a1a.5 transcripts were detected in the pronephric duct, ear, and mucous cells. This expression pattern continues through 108 hpf, when high levels of expression were also detected in the intestinal bulb.