PUBLICATION
NompC TRP channel required for vertebrate sensory hair cell mechanotransduction
- Authors
- Sidi, S., Friedrich, R.W., and Nicolson, T.
- ID
- ZDB-PUB-030715-6
- Date
- 2003
- Source
- Science (New York, N.Y.) 301(5629): 96-99 (Journal)
- Registered Authors
- Friedrich, Rainer, Nicolson, Teresa, Sidi, Samuel
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Phenotype
- Mechanotransduction, Cellular*
- In Situ Hybridization
- Postural Balance
- Deafness
- Molecular Sequence Data
- Ear, Inner/embryology
- Hearing
- Zebrafish
- Hair Cells, Auditory/physiology*
- Oligonucleotides, Antisense
- Cochlear Microphonic Potentials
- Computational Biology
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Endocytosis
- Ion Channels/chemistry
- Ion Channels/genetics
- Ion Channels/physiology*
- Gene Expression
- Reflex, Startle
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- Transient Receptor Potential Channels
- PubMed
- 12805553 Full text @ Science
Citation
Sidi, S., Friedrich, R.W., and Nicolson, T. (2003) NompC TRP channel required for vertebrate sensory hair cell mechanotransduction. Science (New York, N.Y.). 301(5629):96-99.
Abstract
The senses of hearing and balance in vertebrates rely on the sensory hair cells (HCs) of the inner ear. The central element of the HC's transduction apparatus is a mechanically gated ion channel of unknown identity. Here we report that the zebrafish ortholog of Drosophila no mechanoreceptor potential C (nompC), which encodes a transient receptor potential (TRP) channel, is critical for HC mechanotransduction. In zebrafish larvae, nompC is selectively expressed in sensory HCs. Morpholino-mediated removal of nompC function eliminated transduction-dependent endocytosis and electrical responses in HCs, resulting in larval deafness and imbalance. These observations indicate that nompC encodes a vertebrate HC mechanotransduction channel.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping