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ZFIN ID: ZDB-PUB-030527-25
Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos
Raya, A., Kawakami, Y., Rodriguez-Esteban, C., Büscher, D., Koth, C.M., Itoh, T., Morita, M., Raya, R.M., Dubova, I., Bessa, J.G., de la Pompa, J.L., and Izpisúa Belmonte, J.C.
Date: 2003
Source: Genes & Development   17(10): 1213-1218 (Journal)
Registered Authors: Büscher, Dirk, Dubova, Ilir, M.D., Itoh, Tohru, Izpisúa Belmonte, Juan Carlos, Kawakami, Yasuhiko, Koth, Chris, Raya, Angel, Raya, Marina, Rodriguez-Esteban, Concepcion
Keywords: none
MeSH Terms:
  • Animals
  • Body Patterning/physiology*
  • Enhancer Elements, Genetic
  • Gene Expression Regulation/physiology*
  • Hedgehog Proteins
  • Membrane Proteins/physiology*
  • Mice
  • Nodal Protein
  • Organizers, Embryonic/physiology
  • Promoter Regions, Genetic
  • Receptors, Notch
  • Signal Transduction/physiology
  • Situs Inversus/embryology
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Transforming Growth Factor beta/genetics*
  • Transforming Growth Factor beta/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed: 12730123 Full text @ Genes & Dev.
Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, we show that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination. We identify Notch-responsive elements in the Nodal promoter, and unveil a direct relationship between Notch activity and Nodal expression around the node. Our findings provide evidence for a mechanism involving Notch activity that translates an initial symmetry-breaking event into asymmetric gene expression.