ZFIN ID: ZDB-PUB-030210-4
Phenylthiourea disrupts thyroid function in developing zebrafish
Elsalini, O.A. and Rohr, K.B.
Date: 2003
Source: Development genes and evolution   212(12): 593-598 (Journal)
Registered Authors: Elsalini, Osama, Rohr, Klaus
Keywords: none
MeSH Terms:
  • Animals
  • Antithyroid Agents/pharmacology*
  • Metamorphosis, Biological
  • Methimazole/pharmacology
  • Mutation
  • Perchlorates/pharmacology
  • Phenylthiourea/pharmacology*
  • Potassium Compounds/pharmacology
  • Propylthiouracil/pharmacology
  • Thyroid Gland/drug effects*
  • Thyroid Gland/embryology
  • Thyroid Gland/metabolism*
  • Thyroid Hormones/pharmacology
  • Zebrafish/embryology*
  • Zebrafish/growth & development
PubMed: 12536323 Full text @ Dev. Genes Evol.
ABSTRACT
Thyroid hormone (T4) can be detected in thyroid follicles in wild-type zebrafish larvae from 3 days of development, when the thyroid has differentiated. In contrast, embryos or larvae treated with goitrogens ( substances such as methimazole, potassium percholorate, and 6-n-propyl-2 -thiouracil) are devoid of thyroid hormone immunoreactivity. Phenythiourea (PTurea; also commonly known as PTU) is widely used in zebrafish research to suppress pigmentation in developing embryos/fry. PTurea contains a thiocarbamide group that is responsible for goitrogenic activity in methimazole and 6-n-propyl-2-thiouracil. In the present study, we show that commonly used doses of 0.003% PTurea abolish T4 immunoreactivity of the thyroid follicles of zebrafish larvae. As development of the thyroid gland is not affected, these data suggest that PTurea blocks thyroid hormone production. Like other goitrogens, PTurea causes delayed hatching, retardation and malformation of embryos or larvae with increasing doses. At doses of 0.003% PTurea, however, toxic side effects seem to be at a minimum, and the maternal contribution of the hormone might compensate for compromised thyroid function during the first days of development.
ADDITIONAL INFORMATION