PUBLICATION
Expression and splice variant analysis of the zebrafish tcf4 transcription factor
- Authors
- Young, R., Reyes, A., and Allende, M.
- ID
- ZDB-PUB-020912-5
- Date
- 2002
- Source
- Mechanisms of Development 117(1-2): 269-273 (Journal)
- Registered Authors
- Allende, Miguel L., Young, Rodrigo
- Keywords
- Lef/tcf; Wnt; high mobility group box; Danio rerio development; diencephalon; torus semicircularis; pretectum; zona limitans
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Body Patterning/genetics
- Brain/embryology
- Brain/metabolism
- Cloning, Molecular
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Molecular Sequence Data
- Proto-Oncogene Proteins/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Sequence Homology, Amino Acid
- Signal Transduction
- TCF Transcription Factors
- Transcription Factor 7-Like 2 Protein
- Transcription Factors/genetics*
- Wnt Proteins
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- PubMed
- 12204269 Full text @ Mech. Dev.
Citation
Young, R., Reyes, A., and Allende, M. (2002) Expression and splice variant analysis of the zebrafish tcf4 transcription factor. Mechanisms of Development. 117(1-2):269-273.
Abstract
Wnt signalling has been implicated in antero-posterior patterning of the vertebrate embryonic body axis and in a number of other developmental processes. One of the downstream effectors of Wnt signalling is the beta -catenin protein which complexes with members of the Lef/tcf transcription factor family. In the zebrafish, specification of the head has been shown to be dependent on the Tcf3 protein which acts as a repressor of the posteriorizing activity of Wnt (Nature 407 (2000) 913 ). Here, we report the cloning and expression pattern of the zebrafish tcf4 gene. In embryos, we find that the tcf4 gene is highly regulated at the level of RNA splicing such that the variant proteins that are produced contain or lack domains proposed to be essential in repression or activation of transcription. Expression of tcf4 mRNA is first detected in a graded fashion in the anterior brain and subsequently becomes restricted to the dorsal diencephalon and anterior midbrain. There is also transient expression in the anterior rhombomeres of the hindbrain and in the developing gut.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping