ZFIN ID: ZDB-PUB-020805-4
Mammalian germ-line transgenesis by transposition
Dupuy, A.J., Clark, K., Carlson, C.M., Fritz, S., Davidson, A.E., Markley, K.M., Finley, K., Fletcher, C.F., Ekker, S.C., Hackett, P.B., Horn, S., and Largaespada, D.A.
Date: 2002
Source: Proc. Natl. Acad. Sci. USA 99(7): 4495-4499 (Journal)
Registered Authors: Clark, Karl, Ekker, Stephen C., Hackett, Perry B.
Keywords: none
MeSH Terms:
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Transposable Elements*
  • Molecular Sequence Data
  • Mutagenesis, Insertional*
  • Transposases/genetics
  • Transposases/physiology
PubMed: 11904379 Full text @ Proc. Natl. Acad. Sci. USA
Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.