ZFIN ID: ZDB-PUB-020618-6
Differential expression of two somatostatin genes during zebrafish embryonic development
Devos, N., Deflorian, G., Biemar, F., Bortolussi, M., Martial, J.A., Peers, B., and Argenton, F.
Date: 2002
Source: Mechanisms of Development   115(1-2): 133-137 (Journal)
Registered Authors: Argenton, Francesco, Biemar, Frédéric, Bortolussi, Marino, Deflorian, Gianluca, Devos, Nathalie, Martial, Joseph A., Peers, Bernard
Keywords: somatostatin; pancreas; central nervous system; zebrafish; development; in situ hybridisation; masterblind; islet-1
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Axin Protein
  • Base Sequence
  • Brain/embryology
  • Brain/metabolism
  • Central Nervous System/embryology
  • Central Nervous System/metabolism*
  • DNA, Complementary
  • Gene Expression*
  • Gene Expression Profiling
  • Humans
  • Molecular Sequence Data
  • Pancreas/embryology
  • Pancreas/metabolism*
  • Phylogeny
  • Protein Precursors/classification
  • Protein Precursors/genetics*
  • Proteins/genetics
  • Repressor Proteins*
  • Sequence Homology, Amino Acid
  • Somatostatin/classification
  • Somatostatin/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins
PubMed: 12049777 Full text @ Mech. Dev.
We have identified the cDNAs of two new zebrafish preprosomatostatins, PPSS1 and PPSS3, in addition to the previously cloned PPSS2 (Argenton et al., 1999). PPSS1 is the orthologue of mammalian PPSSs, with a conserved C-terminal SS-14 sequence, PPSS2 is a divergent SS precursor and PPSS3 is a cortistatin-like prohormone. Using whole-mount in situ hybridisation, we have analysed the expression of PPSS1 and PPSS2 in zebrafish embryos up to 5 days post fertilisation. PPSS1 was expressed in the developing pancreas and central nervous system (CNS), whereas PPSS2 expression was exclusively pancreatic. In the CNS, PPSS1 was detected in several areas, in particular in the vagal motor nucleus and in cells that pioneer the tract of the postoptic commissure. PPSS1 was also expressed transiently in the telencephalon and spinal motor neurons . In all areas but the telencephalon PPSS1 was coexpressed with islet-1.