Differential expression of two somatostatin genes during zebrafish embryonic development
- Devos, N., Deflorian, G., Biemar, F., Bortolussi, M., Martial, J.A., Peers, B., and Argenton, F.
- Mechanisms of Development 115(1-2): 133-137 (Journal)
- Registered Authors
- Argenton, Francesco, Biemar, Frédéric, Bortolussi, Marino, Deflorian, Gianluca, Devos, Nathalie, Martial, Joseph A., Peers, Bernard
- somatostatin; pancreas; central nervous system; zebrafish; development; in situ hybridisation; masterblind; islet-1
- MeSH Terms
- Amino Acid Sequence
- Axin Protein
- Base Sequence
- Central Nervous System/embryology
- Central Nervous System/metabolism*
- DNA, Complementary
- Gene Expression*
- Gene Expression Profiling
- Molecular Sequence Data
- Protein Precursors/classification
- Protein Precursors/genetics*
- Repressor Proteins*
- Sequence Homology, Amino Acid
- Zebrafish Proteins
- 12049777 Full text @ Mech. Dev.
Devos, N., Deflorian, G., Biemar, F., Bortolussi, M., Martial, J.A., Peers, B., and Argenton, F. (2002) Differential expression of two somatostatin genes during zebrafish embryonic development. Mechanisms of Development. 115(1-2):133-137.
We have identified the cDNAs of two new zebrafish preprosomatostatins, PPSS1 and PPSS3, in addition to the previously cloned PPSS2 (Argenton et al., 1999). PPSS1 is the orthologue of mammalian PPSSs, with a conserved C-terminal SS-14 sequence, PPSS2 is a divergent SS precursor and PPSS3 is a cortistatin-like prohormone. Using whole-mount in situ hybridisation, we have analysed the expression of PPSS1 and PPSS2 in zebrafish embryos up to 5 days post fertilisation. PPSS1 was expressed in the developing pancreas and central nervous system (CNS), whereas PPSS2 expression was exclusively pancreatic. In the CNS, PPSS1 was detected in several areas, in particular in the vagal motor nucleus and in cells that pioneer the tract of the postoptic commissure. PPSS1 was also expressed transiently in the telencephalon and spinal motor neurons . In all areas but the telencephalon PPSS1 was coexpressed with islet-1.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes