PUBLICATION
Zebrafish mutagenesis yields eye morphological mutants with retinal and lens defects
- Authors
- Vihtelic, T.S. and Hyde, D.R.
- ID
- ZDB-PUB-020227-2
- Date
- 2002
- Source
- Vision Research 42(4): 535-540 (Journal)
- Registered Authors
- Hyde, David R., Vihtelic, Thomas
- Keywords
- zebrafish; Danio rerio; mutagenesis; retina; lens; programmed cell death
- MeSH Terms
-
- Animals
- Apoptosis
- Ethylnitrosourea
- Eye/embryology*
- Eye/pathology
- Eye Color
- Eye Diseases/embryology
- Eye Diseases/genetics*
- Eye Diseases/pathology
- Immunohistochemistry
- Lens, Crystalline/pathology
- Models, Animal*
- Mutagenesis
- Retina/embryology
- Retinal Degeneration/embryology
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 11853770 Full text @ Vision Res.
Citation
Vihtelic, T.S. and Hyde, D.R. (2002) Zebrafish mutagenesis yields eye morphological mutants with retinal and lens defects. Vision Research. 42(4):535-540.
Abstract
A chemical mutagenesis to identify zebrafish eye morphological mutants was performed by screening F(3) larvae at 5 and 7 days post-fertilization (dpf) for changes in eye or pupil size. Based on histological analysis, four different phenotypic classes were obtained. The two Class I and three Class II mutants are all characterized by small eyes and exhibit defects in early retinal development or unregulated cell death, respectively. The single Class III mutant has reduced ocular pigmentation. The three Class IV mutants display defects in the ocular lens, including one mutant line with normal sized eyes and pupils that develops lens opacity at 7 dpf.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping