PUBLICATION
Green fluorescent protein (GFP) transgenic fish and their applications
- Authors
- Gong, Z., Ju, B., and Wan, H.
- ID
- ZDB-PUB-020219-8
- Date
- 2001
- Source
- Genetica 111(1-3): 213-225 (Journal)
- Registered Authors
- Gong, Zhiyuan, Ju, Bensheng, Wan, Haiyan
- Keywords
- biomonitoring, dsRed, embryo, enhancer, medaka, ornamental fish, promoter, rainbow trout, RFP, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- Cell Lineage
- Cell Movement
- Chimera
- Enhancer Elements, Genetic
- Fishes/embryology
- Fishes/genetics*
- Green Fluorescent Proteins
- Luminescent Proteins/genetics*
- Mutagenesis
- Promoter Regions, Genetic
- PubMed
- 11841167 Full text @ Genetica
Citation
Gong, Z., Ju, B., and Wan, H. (2001) Green fluorescent protein (GFP) transgenic fish and their applications. Genetica. 111(1-3):213-225.
Abstract
The coupling of the GFP reporter system with the optical clarity of embryogenesis in model fish such as zebrafish and medaka is beginning to change the picture of transgenic fish study. Since the advent of first GFP transgenic fish in 1995, GFP transgenic fish technology have been quickly employed in many areas such as analyses of gene expression patterns and tissue/organ development, dissection of promoters/enhancers, cell lineage and axonal pathfinding, cellular localization of protein products, chimeric embryo and nuclear transplantation, cell sorting, etc. The GFP transgenic fish also have the potentials in analysis of upstream regulatory factors, mutagenesis screening and characterization, and promoter/enhancer trap. Our own studies indicate that GFP transgenic fish may become a new source of novel variety of ornamental fish. Efforts are also being made in our laboratory to turn GFP transgenic fish into biomonitoring organisms for surveillance of environmental pollution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping