PUBLICATION

Acetylcholinesterase is required for neuronal and muscular development in the zebrafish embryo

Authors
Behra, M., Cousin, X., Bertrand, C., Vonesch, J.L., Biellmann, D., Chatonnet, A., and Strähle, U.
ID
ZDB-PUB-020107-3
Date
2002
Source
Nature Neuroscience   5(2): 111-118 (Journal)
Registered Authors
Behra, Martine, Bertrand, Christelle, Biellmann, Dominique, Chatonnet, Arnaud, Cousin, Xavier, Strähle, Uwe
Keywords
none
MeSH Terms
  • Acetylcholinesterase/genetics
  • Acetylcholinesterase/metabolism
  • Acetylcholinesterase/physiology*
  • Amino Acid Sequence/genetics
  • Animals
  • Base Sequence/genetics
  • Cell Death
  • Embryo, Nonmammalian/physiology
  • Molecular Sequence Data
  • Muscle, Skeletal/embryology*
  • Muscular Diseases/genetics
  • Mutation/physiology
  • Nervous System/embryology*
  • Neuromuscular Junction/embryology
  • Neurons/physiology*
  • Neurons, Afferent/physiology
  • Phenotype
  • Receptors, Nicotinic/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
11753420 Full text @ Nat. Neurosci.
Abstract
The neurotransmitter acetylcholine (ACh) has a crucial role in central and neuromuscular synapses of the cholinergic system. After release into the synaptic cleft, ACh is rapidly degraded by acetylcholinesterase (AChE). We have identified a mutation in the ache gene of the zebrafish, which abolishes ACh hydrolysis in homozygous animals completely. Embryos are initially motile but subsequently develop paralysis. Mutant embryos show defects in muscle fiber formation and innervation, and primary sensory neurons die prematurely. The neuromuscular phenotype in ache mutants is suppressed by a homozygous loss-of-function allele of the alpha-subunit of the nicotinic acetylcholine receptor (nAChR), indicating that the impairment of neuromuscular development is mediated by activation of nAChR in the mutant. Here we provide genetic evidence for non-classical functions of AChE in vertebrate development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes