ZFIN ID: ZDB-PUB-010706-8
Expression of zebrafish btg-b, an anti-proliferative cofactor, during early embryogenesis
Sakaguchi, T., Kuroiwa, A., and Takeda, H.
Date: 2001
Source: Mechanisms of Development   104(1-2): 113-115 (Journal)
Registered Authors: Sakaguchi, Takuya, Takeda, Hiroyuki
Keywords: zebrafish; btg-b; Btg2; Hoxb9
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain/metabolism
  • Carrier Proteins/biosynthesis*
  • Carrier Proteins/genetics*
  • Cell Cycle/genetics*
  • Cloning, Molecular
  • DNA, Complementary/metabolism
  • Embryo, Nonmammalian/metabolism
  • Gastrula/metabolism
  • Gene Library
  • In Situ Hybridization
  • Mesoderm/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Protein Structure, Tertiary
  • Radiation Hybrid Mapping
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish Proteins*
PubMed: 11404086 Full text @ Mech. Dev.
BTG/tob family proteins are thought to be a potential tumor suppressor due to their anti-proliferative activity. We cloned zebrafish btg-b, a member of the BTG1/2 subfamily, using in situ hybridization screening. The tissue-specific expression of btg-b is first observed in the organizer region at the early gastrula stage. Later in development, the forebrain, the hindbrain, the polster and the paraxial mesoderm transiently express btg-b. Recently, mouse Btg1 and Btg2 have been shown to be a cofactor for Hoxb9. Double in situ hybridization with zebrafish btg-b and hoxb9a indicates that the expression domains of these two genes overlap in the posterior paraxial mesoderm.