PUBLICATION
Zebrafish homolog of the leukemia gene CBFB: its expression during embryogenesis and its relationship to scl and gata-1 in hematopoiesis
- Authors
- Blake, T., Adya, N., Kim, C.H., Oates, A.C., Zon, L., Chitnis, A., Weinstein, B.M., and Liu, P.P.
- ID
- ZDB-PUB-001221-16
- Date
- 2000
- Source
- Blood 96(13): 4178-4184 (Journal)
- Registered Authors
- Chitnis, Ajay, Kim, Cheol-Hee, Liu, Pu Paul, Oates, Andrew, Weinstein, Brant M., Zon, Leonard I.
- Keywords
- none
- MeSH Terms
-
- Leukemia/genetics*
- Humans
- Animals
- Chromosomes, Human, Pair 16/genetics*
- DNA/metabolism
- Erythroid-Specific DNA-Binding Factors
- DNA, Complementary/genetics
- Embryo, Nonmammalian/metabolism
- Molecular Sequence Data
- Basic Helix-Loop-Helix Transcription Factors
- Transcription Factor AP-2
- Chromosome Inversion*
- Proto-Oncogene Proteins*
- Core Binding Factor beta Subunit
- Kidney/chemistry
- Genes
- Amino Acid Sequence
- Species Specificity
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins*
- Core Binding Factor alpha Subunits
- Organ Specificity
- Mice
- Protein Binding
- GATA1 Transcription Factor
- Sequence Homology, Amino Acid
- Gene Expression Regulation, Developmental*
- Hematopoiesis/genetics*
- Sequence Alignment
- DNA-Binding Proteins/biosynthesis
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism
- Transcription Factors/biosynthesis
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Mesoderm/metabolism
- PubMed
- 11110689
Citation
Blake, T., Adya, N., Kim, C.H., Oates, A.C., Zon, L., Chitnis, A., Weinstein, B.M., and Liu, P.P. (2000) Zebrafish homolog of the leukemia gene CBFB: its expression during embryogenesis and its relationship to scl and gata-1 in hematopoiesis. Blood. 96(13):4178-4184.
Abstract
Mammalian CBFB encodes a transcription factor (CBFbeta) that in combination with CBFalpha2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. CBFB is associated with human leukemias through a chromosome 16 inversion and is essential for definitive hematopoiesis during mouse embryo development. We have isolated a zebrafish cbfb complementary DNA (cDNA) clone from a zebrafish kidney cDNA library. This cbfb is highly homologous to human and mouse CBFB/Cbfb genes at both the DNA and protein level. In biochemical analyses, cbfbeta binds to human CBFalpha2 and enhances its DNA binding. During zebrafish development, cbfb is expressed in the lateral plate mesoderm at tail bud stage and in the intermediate cell mass (ICM, the location of embryonic hematopoiesis) between the 21- to 26-somite stages. The cbfb is also expressed in Rohon-Beard cells, cranial nerve ganglia, hindbrain, retina, branchial arches, jaw, and fin buds. Expression of cbfb is decreased or absent in the ICM and Rohon-Beard cells in some hematopoietic mutants and is unaffected in others. We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. Our results indicate that cbfb is expressed in early hematopoietic progenitors and that its expression pattern in the hematopoietic mutants is similar to that of scl. (Blood. 2000;96:4178-4184)
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping