ZFIN ID: ZDB-LAB-111101-1
Clark Lab
PI/Director: Clark, Karl
Contact Person: Clark, Karl
Email: clark.karl@mayo.edu
URL: http://mayoresearch.mayo.edu/mayo/research/staff/Clark_KJ.cfm
Address: Dept of Biochemistry and Molecular Biology Guggenheim 13, Mayo Clinic 221 4th Ave. SW Rochester MN 55902 USA
Country: United States
Phone: 507-538-5319
Fax: 507-293-1058
Line Designation: mn


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 1 genomic features


STATEMENT OF RESEARCH INTERESTS
Our lab is focused on applying molecular and genetic tools to dissect and discover the interactions between genes and the environment that affect both short and long-term changes in physiology and behavior. In particular, We are interested in the bridge between genes and environment that is gated by the stress response.


LAB MEMBERS
Krug, Randall G. Graduate Student Lee, Han B. Graduate Student Poshusta, Tanya Technical Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
El-Rass, S., Eisa-Beygi, S., Khong, E., Brand-Arzamendi, K., Mauro, A., Zhang, H., Clark, K.J., Ekker, S.C., Wen, X.Y. (2017) Disruption of pdgfra alters endocardial and myocardial fusion during zebrafish cardiac assembly. Biology Open.
Gerlai, R., Poshusta, T.L., Rampersad, M., Fernandes, Y., Greenwood, T.M., Cousin, M.A., Klee, E.W., Clark, K.J. (2016) Forward Genetic Screening Using Behavioral Tests in Zebrafish: A Proof of Concept Analysis of Mutants. Behavior Genetics. 47(1):125-139
Ata, H., Clark, K.J., Ekker, S.C. (2016) The zebrafish genome editing toolkit. Methods in cell biology. 135:149-70
Leveque, R.E., Clark, K.J., Ekker, S.C. (2016) Mayo Clinic Zebrafish Facility Overview. Zebrafish. 13 Suppl 1:S44-6
Ma, A.C., McNulty, M.S., Tanya, P.L., Jarryd, C.M., Martínez-Gálvez, G., Argue, D.P., Lee, H.B., Urban, M.D., Bullard, C.E., Blackburn, P.R., Man, T.K., Clark, K.J., Ekker, S.C. (2016) FusX: A rapid one-step TALE assembly system for genome science. Human gene therapy. 27(6):451-63
Krug, R.G., Poshusta, T.L., Skuster, K.J., Berg, M.R., Gardner, S.L., Clark, K.J. (2014) A transgenic zebrafish model for monitoring glucocorticoid receptor activity. Genes, brain, and behavior. 13(5):478-87
Ma, A.C., Lee, H.B., Clark, K.J., and Ekker, S.C. (2013) High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD GoldyTALEN Design. PLoS One. 8(5):e65259
Blackburn, P.R., Campbell, J.M., Clark, K.J., and Ekker, S.C. (2013) The CRISPR System-Keeping Zebrafish Gene Targeting Fresh. Zebrafish. 10(1):116-8
Patowary, A., Purkanti, R., Singh, M., Chauhan, R., Singh, A.R., Swarnkar, M., Singh, N., Pandey, V., Torroja, C., Clark, M.D., Kocher, J.P., Clark, K.J., Stemple, D.L., Klee, E.W., Ekker, S.C., Scaria, V., and Sivasubbu, S. (2013) A sequence-based variation map of zebrafish. Zebrafish. 10(1):15-20
Ding, Y., Liu, W., Deng, Y., Jomok, B., Yang, J., Huang, W., Clark, K.J., Zhong, T., Lin, X., Ekker, S.C., and Xu, X. (2013) Trapping Cardiac Recessive Mutants via Expression-based Insertional Mutagenesis Screening. Circulation research. 112(4):606-617
Bedell, V.M., Wang, Y., Campbell, J.M., Poshusta, T.L., Starker, C.G., Krug, R.G., Tan, W., Penheiter, S.G., Ma, A.C., Leung, A.Y., Fahrenkrug, S.C., Carlson, D.F., Voytas, D.F., Clark, K.J., Essner, J.J., and Ekker, S.C. (2012) In vivo genome editing using a high-efficiency TALEN system. Nature. 491(7422):114-118
Hoeppner, L.H., Phoenix, K.N., Clark, K.J., Bhattacharya, R., Gong, X., Sciuto, T.E., Vohra, P., Suresh, S., Bhattacharya, S., Dvorak, A.M., Ekker, S.C., Dvorak, H.F., Claffey, K.P., and Mukhopadhyay, D. (2012) Revealing the role of phospholipase Cβ3 in the regulation of VEGF-induced vascular permeability. Blood.. 120(11):2167-2173
Bedell, V.M., Person, A.D., Larson, J.D., McLoon, A., Balciunas, D., Clark, K.J., Neff, K.I., Nelson, K.E., Bill, B.R., Schimmenti, L.A., Beiraghi, S., and Ekker, S.C. (2012) The lineage-specific gene ponzr1 is essential for zebrafish pronephric and pharyngeal arch development. Development (Cambridge, England). 139(4):793-804
Xu, J., Gao, J., Li, J., Xue, L., Clark, K.J., Ekker, S.C., and Du, S.J. (2012) Functional analysis of slow Myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow muscles. Journal of Genetic Genomics. 39(2):69-80
Ekker, S., Clark, K., ZFIN Staff (2012) Curation of zfishbook links. ZFIN Direct Data Submission.
Klee, E.W., Schneider, H., Clark, K.J., Cousin, M.A., Ebbert, J.O., Hooten, W.M., Karpyak, V.M., Warner, D.O., and Ekker, S.C. (2011) Zebrafish: a model for the study of addiction genetics. Human genetics. 131(6):977-1008
Liao, H.K., Wang, Y., Watt, K.E., Wen, Q., Breitback, J., Kemmet, C.K., Clark, K.J., Ekker, S.C., Essner, J.J., and McGrail, M. (2011) Tol2 gene trap integrations in the zebrafish amyloid precursor protein genes appa and aplp2 reveal accumulation of secreted APP at the embryonic veins. Developmental dynamics : an official publication of the American Association of Anatomists. 241(2):415-425
Clark, K.J., Voytas, D.F., and Ekker, S.C. (2011) A TALE of Two Nucleases: Gene Targeting for the Masses. Zebrafish. 8(3):147-149
Clark, K.J., Balciunas, D., Pogoda, H.M., Ding, Y., Westcot, S.E., Bedell, V.M., Greenwood, T.M., Urban, M.D., Skuster, K.J., Petzold, A.M., Ni, J., Nielsen, A.L., Patowary, A., Scaria, V., Sivasubbu, S., Xu, X., Hammerschmidt, M., and Ekker, S.C. (2011) In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Nature Methods. 8(6):506-512
Clark, K.J., Boczek, N.J., and Ekker, S.C. (2011) Stressing zebrafish for behavioral genetics. Reviews in the Neurosciences. 22(1):49-62
Clark, K.J., Urban, M.D., Skuster, K.J., and Ekker, S.C. (2011) Transgenic zebrafish using transposable elements. Meth. Cell. Biol.. 104:137-149
Wang, Y., Kaiser, M.S., Larson, J.D., Nasevicius, A., Clark, K.J., Wadman, S.A., Roberg-Perez, S.E., Ekker, S.C., Hackett, P.B., McGrail, M., and Essner, J.J. (2010) Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis. Development (Cambridge, England). 137(18):3119-3128
Petzold, A.M., Bedell, V.M., Boczek, N.J., Essner, J.J., Balciunas, D., Clark, K.J., and Ekker, S.C. (2010) SCORE imaging: specimen in a corrected optical rotational enclosure. Zebrafish. 7(2):149-154
Petzold, A.M., Balciunas, D., Sivasubbu, S., Clark, K.J., Bedell, V.M., Westcot, S.E., Myers, S.R., Moulder, G.L., Thomas, M.J., and Ekker, S.C. (2009) Nicotine response genetics in the zebrafish. Proc. Natl. Acad. Sci. USA. 106(44):18662-18667
Kalén, M., Wallgard, E., Asker, N., Nasevicius, A., Athley, E., Billgren, E., Larson, J.D., Wadman, S.A., Norseng, E., Clark, K.J., He, L., Karlsson-Lindahl, L., Häger, A.K., Weber, H., Augustin, H., Samuelsson, T., Kemmet, C.K., Utesch, C.M., Essner, J.J., Hackett, P.B., and Hellström, M. (2009) Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets. Chemistry & Biology. 16(4):432-441
Bill, B.R., Petzold, A.M., Clark, K.J., Schimmenti, L.A., and Ekker, S.C. (2009) A primer for morpholino use in zebrafish. Zebrafish. 6(1):69-77
Larson, J.D., Wadman, S.A., Chen, E., Kerley, L., Clark, K.J., Eide, M., Lippert, S., Nasevicius, A., Ekker, S.C., Hackett, P.B., and Essner, J.J. (2004) Expression of VE-cadherin in zebrafish embryos: A new tool to evaluate vascular development. Developmental dynamics : an official publication of the American Association of Anatomists. 231(1):204-213
Clark, K.J., Geurts, A.M., Bell, J.B., and Hackett, P.B. (2004) Transposon vectors for gene-trap insertional mutagenesis in vertebrates. Genesis (New York, N.Y. : 2000). 39(4):225-233
Dupuy, A.J., Clark, K., Carlson, C.M., Fritz, S., Davidson, A.E., Markley, K.M., Finley, K., Fletcher, C.F., Ekker, S.C., Hackett, P.B., Horn, S., and Largaespada, D.A. (2002) Mammalian germ-line transgenesis by transposition. Proc. Natl. Acad. Sci. USA. 99(7):4495-4499
Cui, Z., Clark, K.J., Kaufman, C.D., and Hackett, P.B. (2001) Inhibition of skiA and skiB gene expression ventralizes zebrafish embryos. Genesis (New York, N.Y. : 2000). 30(3):149-153
Fahrenkrug, S.C., Dahlquist, M.O., Clark, K.J., and Hackett, P.B. (1999) Dynamic and tissue-specific expression of elF4E during zebrafish embryogenesis. Differentiation; research in biological diversity. 65(4):191-201
Fahrenkrug, S.C., Clark, K., Dahlquist, M.O., and Hackett, P.B. (1999) Dicistronic gene expression in developing zebrafish. Mar. Biotechnol.. 1(6):552-561