ZFIN ID: ZDB-LAB-050811-1
Sagasti lab
PI/Director: Sagasti, Alvaro
Contact Person: Sagasti, Alvaro
Email: sagasti@mcdb.ucla.edu
URL: http://www.mcdb.ucla.edu/Research/Sagasti/
Address: Department of MCD Biology, UCLA Life Science Building 4226 P.O. Box 951606 Los Angeles, CA 90095-1606
Country: United States
Phone:
Fax:
Line Designation: la


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 28 genomic features


STATEMENT OF RESEARCH INTERESTS
We’re interested in how nerve cells adopt their complex shapes. During development neurons must elaborate intricate axonal and dendritic processes to make contact with their environment or with other nerve cells. Proper development of these contacts is crucial for animals to sense their environment and respond appropriately.

As a model for the development of complex cell shapes in the nervous system, we are studying trigeminal neurons in zebrafish. Trigeminal neurons sense painful and benign mechanical and thermal stimuli in the skin. They are ideal for the study of cell shape because they possess complex peripheral axons that arborize extensively under the skin and can easily be accessed and imaged. We employ a combination of embryological, genetic, molecular, imaging and behavioral approaches to address these questions.


LAB MEMBERS
Wang, Fang Post-Doc O'Brien, Georgeann Graduate Student Porter, Michael Research Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Yang, Q., Wu, F., Mi, Y., Wang, F., Cai, K., Yang, X., Zhang, R., Liu, L., Zhang, Y., Wang, Y., Wang, X., Xu, M., Gui, Y., Li, Q. (2020) Aberrant expression of miR-29b-3p influences heart development and cardiomyocyte proliferation by targeting NOTCH2. Cell Proliferation. 53(3):e12764
Cui, J., Wang, F., Gao, J., Zhai, W., Zhou, Z., Liu, D., Wang, P. (2019) Bioaccumulation and metabolism of carbosulfan in zebrafish Danio rerio and toxic effects with its metabolites. Journal of Agricultural and Food Chemistry. 67(45):12348-12356
Feng, C., Thyagarajan, P., Shorey, M., Seebold, D.Y., Weiner, A.T., Albertson, R.M., Rao, K.S., Sagasti, A., Goetschius, D.J., Rolls, M.M. (2019) Patronin-mediated minus end growth is required for dendritic microtubule polarity. The Journal of cell biology. 218(7):2309-2328
Jiang, N., Rasmussen, J.P., Clanton, J.A., Rosenberg, M.F., Luedke, K.P., Cronan, M.R., Parker, E.D., Kim, H.J., Vaughan, J.C., Sagasti, A., Parrish, J.Z. (2019) A conserved morphogenetic mechanism for epidermal ensheathment of nociceptive sensory neurites. eLIFE. 8:
Julien, D.P., Chan, A.W., Barrios, J., Mathiaparanam, J., Douglass, A., Wolman, M.A., Sagasti, A. (2018) Zebrafish expression reporters and mutants reveal that the IgSF cell adhesion molecule Dscamb is required for feeding and survival. Journal of neurogenetics. 32(4):336-352
Rasmussen, J.P., Vo, N.T., Sagasti, A. (2018) Fish Scales Dictate the Pattern of Adult Skin Innervation and Vascularization. Developmental Cell. 46(3):344-359.e4
Wang, Y., Zhang, H., Lu, Y., Wang, F., Liu, L., Liu, J., Liu, X. (2017) Comparative transcriptome analysis of zebrafish (Danio rerio) brain and spleen infected with spring viremia of carp virus (SVCV). Fish & shellfish immunology. 69:35-45
Madigan, C.A., Cambier, C.J., Kelly-Scumpia, K.M., Scumpia, P.O., Cheng, T.Y., Zailaa, J., Bloom, B.R., Moody, D.B., Smale, S.T., Sagasti, A., Modlin, R.L., Ramakrishnan, L. (2017) A Macrophage Response to Mycobacterium leprae Phenolic Glycolipid Initiates Nerve Damage in Leprosy. Cell. 170:973-985.e10
Rasmussen, J.P., Sagasti, A. (2017) Learning to swim, again: Axon regeneration in fish. Experimental neurology. 287(Pt 3):318-330
Xiao, C., Gao, L., Hou, Y., Xu, C., Chang, N., Wang, F., Hu, K., He, A., Luo, Y., Wang, J., Peng, J., Tang, F., Zhu, X., Xiong, J.W. (2016) Chromatin-remodelling factor Brg1 regulates myocardial proliferation and regeneration in zebrafish. Nature communications. 7:13787
Lulla, A., Barnhill, L., Bitan, G., Ivanova, M.I., Nguyen, B., O'Donnell, K., Stahl, M.C., Yamashiro, C., Klärner, F.G., Schrader, T., Sagasti, A., Bronstein, J.M. (2016) Neurotoxicity of the Parkinson's Disease-Associated Pesticide Ziram Is Synuclein-Dependent in Zebrafish Embryos. Environmental health perspectives. 124(11):1766-1775
Vargas, M.E., Yamagishi, Y., Tessier-Lavigne, M., Sagasti, A. (2015) Live Imaging of Calcium Dynamics during Axon Degeneration Reveals Two Functionally Distinct Phases of Calcium Influx. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35:15026-38
Wang, B., Liu, Y., Sun, Z., Liu, X., Mi, Y., Liu, D., Xu, X., Hou, Q., Wang, F., Hu, C. (2015) A splicing isoform of Ctenopharyngodon idella ADAR1 (CiADAR1-like): Genome organization, tissue specific expression and transcriptional regulation. Fish & shellfish immunology. 47:535-544
Zhang, C., Zhang, Q., Wang, F., Liu, Q. (2015) Knockdown of poc1b causes abnormal photoreceptor sensory cilia and vision impairment in zebrafish. Biochemical and Biophysical Research Communications. 465(4):651-7
Wu, F., Song, H., Zhang, Y., Zhang, Y., Mu, Q., Jiang, M., Wang, F., Zhang, W., Li, L., Li, H., Wang, Y., Zhang, M., Li, S., Yang, L., Meng, Y., Tang, D. (2015) Irisin Induces Angiogenesis in Human Umbilical Vein Endothelial Cells In Vitro and in Zebrafish Embryos In Vivo via Activation of the ERK Signaling Pathway. PLoS One. 10:e0134662
Sun, Z., Wang, B., Liu, Y., Liu, X., Mi, Y., Gu, M., Wang, F., Wu, C., Hu, C. (2015) Genome organization and transcriptional regulation of Adenosine Deaminase Acting on RNA gene 1 (ADAR1) in grass carp (Ctenopharyngodon idella). Developmental and comparative immunology. 50(2):98-105
Rasmussen, J.P., Sack, G.S., Martin, S.M., Sagasti, A. (2015) Vertebrate epidermal cells are broad-specificity phagocytes that clear sensory axon debris. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35:559-70
Julien, D.P., Sagasti, A. (2014) Synaptic Specificity: When the Neighbors Are Away, Sensory Axons Turn Promiscuous. Current biology : CB. 24:R1168-R1170
Chun, R.F., Blatter, E., Elliott, S., Fitz-Gibbon, S., Rieger, S., Sagasti, A., Adams, J.S., Hewison, M. (2014) Cloning of a functional 25-hydroxyvitamin D-1α-hydroxylase in zebrafish (Danio rerio). Cell biochemistry and function. 32(8):675-82
Wang, G., Wang, F., Cao, D., Liu, Y., Zhang, R., Ye, H., Li, X., He, L., Yang, Z., Ma, L., Peng, A., Xiang, M., Wei, Y., Chen, L. (2014) Synthesis, structure-activity relationships and biological evaluation of barbigerone analogues as anti-proliferative and anti-angiogenesis agents. Bioorganic & medicinal chemistry letters. 24(14):3158-63
O'Donnell, K.C., Lulla, A., Stahl, M.C., Wheat, N.D., Bronstein, J.M., Sagasti, A. (2014) Axon degeneration and PGC-1alpha-mediated protection in a zebrafish model of alpha-synuclein toxicity. Disease models & mechanisms. 7:571-82
O'Donnell, K.C., Vargas, M.E., and Sagasti, A. (2013) WldS and PGC-1alpha Regulate Mitochondrial Transport and Oxidation State after Axonal Injury. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(37):14778-14790
Zhu, Y., Wang, D., Wang, F., Li, T., Dong, L., Liu, H., Ma, Y., Jiang, F., Yin, H., Yan, W., Luo, M., Tang, Z., Zhang, G., Wang, Q., Zhang, J., Zhou, J., and Yu, J. (2013) A comprehensive analysis of GATA-1-regulated miRNAs reveals miR-23a to be a positive modulator of erythropoiesis. Nucleic acids research. 41(7):4129-43
Palanca, A.M., Lee, S.L., Yee, L.E., Joe-Wong, C., Trinh, L.A., Hiroyasu, E., Husain, M., Fraser, S.E., Pellegrini, M., and Sagasti, A. (2013) New transgenic reporters identify somatosensory neuron subtypes in larval zebrafish. Developmental Neurobiology. 73(2):152-167
Palanca, A.M., and Sagasti, A. (2013) Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato. Journal of visualized experiments : JoVE. (71):e50184
Fitzmaurice, A.G., Rhodes, S.L., Lulla, A., Murphy, N.P., Lam, H.A., O'Donnell, K.C., Barnhill, L., Casida, J.E., Cockburn, M., Sagasti, A., Stahl, M.C., Maidment, N.T., Ritz, B., and Bronstein, J.M. (2013) Aldehyde dehydrogenase inhibition as a pathogenic mechanism in Parkinson disease. Proceedings of the National Academy of Sciences of the United States of America. 110(2):636-641
Issa, F.A., Mock, A.F., Sagasti, A., and Papazian, D.M. (2012) Spinocerebellar ataxia type 13 mutation associated with disease onset in infancy disrupts axonal pathfinding during neuronal development. Disease models & mechanisms. 5(6):921-929
Villegas, R., Martin, S.M., O'Donnell, K., Carrillo, S., Sagasti, A., and Allende, M.L. (2012) Dynamics of degeneration and regeneration in developing zebrafish peripheral axons reveals a requirement for extrinsic cell types. Neural Development. 7(1):19
Wang, F., Wolfson, S.N., Gharib, A., and Sagasti, A. (2012) LAR Receptor Tyrosine Phosphatases and HSPGs Guide Peripheral Sensory Axons to the Skin. Current biology : CB. 22(5):373-382
O'Brien, G.S., Rieger, S., Wang, F., Smolen, G.A., Gonzalez, R.E., Buchanan, J., and Sagasti, A. (2012) Coordinate development of skin cells and cutaneous sensory axons in zebrafish. The Journal of comparative neurology. 520(4):816-31
Rieger, S., Wang, F., and Sagasti, A. (2011) Time-lapse imaging of neural development: Zebrafish lead the way into the fourth dimension. Genesis (New York, N.Y. : 2000). 49(7):534-45
Rieger, S., and Sagasti, A. (2011) Hydrogen peroxide promotes injury-induced peripheral sensory axon regeneration in the zebrafish skin. PLoS Biology. 9(5):e1000621
Issa, F.A., O'Brien, G., Kettunen, P., Sagasti, A., Glanzman, D.L., Papazian, D.M. (2011) Neural circuit activity in freely behaving zebrafish (Danio rerio). The Journal of experimental biology. 213(Pt 6):1028-1038
Martin, S.M., O'Brien, G.S., Portera-Cailliau, C., and Sagasti, A. (2010) Wallerian degeneration of zebrafish trigeminal axons in the skin is required for regeneration and developmental pruning. Development (Cambridge, England). 137(23):3985-3994
O'Brien, G.S., Martin, S.M., Söllner, C., Wright, G.J., Becker, C.G., Portera-Cailliau, C., and Sagasti, A. (2009) Developmentally Regulated Impediments to Skin Reinnervation by Injured Peripheral Sensory Axon Terminals. Current biology : CB. 19(24):2086-2090
Sagasti, A., O'Brien, G.S., Rieger, S., Martin, S.M., Cavanaugh, A.M., and Portera-Cailliau, C. (2009) Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos. Journal of visualized experiments : JoVE. (24)
Merchant, S.S., and Sagasti, A. (2006) Precious metal economy. Cell Metabolism. 4(2):99-101
Sagasti, A., Guido, M.R., Raible, D.W., and Schier, A.F. (2005) Repulsive interactions shape the morphologies and functional arrangement of zebrafish peripheral sensory arbors. Current biology : CB. 15(9):804-814