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Research
General Information
ZIRC
ZFIN ID: ZDB-LAB-000918-2
A. Thomas Look Lab
PI/Director: Look, A. Thomas
Contact Person: He, Shuning
Email: shuning_he@dfci.harvard.edu
URL: http://research.dfci.harvard.edu/looklab/
Address: Department of Pediatric Oncology Dana-Farber Cancer Institute Harvard Medical School Mayer Building 630 450 Brookline Ave Boston, MA 02215 USA
Country: United States
Phone: (617) 632-5826
Fax: (617) 632-6989
Line Designation: zdf


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 34 genomic features


STATEMENT OF RESEARCH INTERESTS
We are studying cancer genetics using the zebrafish animal model, in combination with murine and cell culture systems, to dissect developmental pathways subverted in human leukemias and solid tumors. The zebrafish model is an established system for studying vertebrate embryogenesis, organogenesis and disease. A powerful attribute of the zebrafish is its capacity for performing large-scale forward genetic screens on transparent, readily accessible embryos. Thus, the zebrafish is an ideal system for identifying novel genes involved in cancer, as their discovery is based on unbiased phenotypic assays and can uncover genes that are either activated (oncogenes) or inactivated (tumor suppressors) during malignant transformation. Using genome-wide mutagenesis screening strategies and TILLING in conjunction with transgenic approaches we are generating zebrafish models of several different cancers.

Zebrafish myelopoiesis is very similar to that in humans and we postulate that through our mutagenesis screens we will identify a subset of genes that both regulates normal myelopoiesis and contributes to the pathogenesis of two important human diseases - myelodysplastic syndrome and acute myeloid leukemia. A second screen is underway to uncover genes disrupted in neuroblastoma, the most common extra-cranial solid tumor of children. These embryonic tumors arise in the peripheral sympathetic nervous system (PSNS) and most of the genes regulating both PSNS development and neuroblastoma formation have yet to be identified. Through these screens we have identified new genes required for normal myelopoiesis and PSNS development and are currently studying their respective contributions to neoplastic pathogenesis. Cell death mechanisms also contribute to malignant transformation and p53, a critical regulator of DNA repair and apoptosis, is the most commonly mutated gene among all cancers. We have recently isolated a mutant p53 zebrafish line that is tumor-prone and is currently being used in conjunction with our zebrafish cancer models to determine how p53 contributes to tissue-specific tumors.

We have shown that human T-cell leukemias can be divided into five major subtypes based on the expression of oncogenes that initiate malignant transformation, and in addition, have generated transgenic zebrafish lines overexpressing Myc that develop leukemia, recapitulating one of these human T-ALL subtypes. We are now conducting one of the first “cancer-related” modifier screens in a vertebrate system to identify both enhancers and suppressors of T-cell leukemia. Chemical and genetic modifier screens using tumor-prone zebrafish lines may ultimately reveal mutant genes or drugs that can suppress or modify disease progression. We hope to identify such modifiers that can either promote specific aspects of the malignancy, such as genomic instability or metastasis, or that delays/suppresses tumor onset. We are also utilizing innovative in vitro genome-scale location analysis (GSLA), combining chromatin immunoprecipitation with hybridization to human promoter microarrays to identify the direct targets of prevalent oncogenic transcription factors. We are validating the significance of key subsets of candidate targets through siRNA strategies and assessing whether their functional inhibition retards aberrant cell growth and survival in panels of cell lines derived from human cancers. Through the combination of these approaches we hope to uncover novel genes and targets for the development of small molecule inhibitors and new cancer therapies.


LAB MEMBERS
Etchin, Julia Post-Doc He, Shuning Post-Doc Jing, Chang-Bin Post-Doc
Ki, Dong Hyuk Post-Doc Li, Zhaodong Post-Doc Oppel, Felix Post-Doc
Shi, Hui Post-Doc Tao, Ting Post-Doc Weichert, Nina Post-Doc
Zimmerman, Mark Post-Doc Debiasi, Daniel Fish Facility Staff Layden, Hillary Fish Facility Staff
Thurston, Grace Fish Facility Staff Berezovskaya, Alla Technical Staff Ormenaj, Myzafere Administrative Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
Gjini, E., Jing, C.B., Nguyen, A.T., Reyon, D., Gans, E., Kesarsing, M., Peterson, J., Pozdnyakova, O., Rodig, S.J., Mansour, M.R., Joung, K., Look, A.T. (2019) Disruption of asxl1 results in myeloproliferative neoplasms in zebrafish. Disease models & mechanisms. 12(5):
Oppel, F., Tao, T., Shi, H., Ross, K.N., Zimmerman, M.W., He, S., Tong, G., Aster, J.C., Look, A.T. (2019) Loss of atrx cooperates with p53-deficiency to promote the development of sarcomas and other malignancies. PLoS Genetics. 15:e1008039
Ma, Z., Zhu, P., Shi, H., Guo, L., Zhang, Q., Chen, Y., Chen, S., Zhang, Z., Peng, J., Chen, J. (2019) PTC-bearing mRNA elicits a genetic compensation response via Upf3a and COMPASS components. Nature. 568(7751):259-263
Henderson, F., Johnston, H.R., Badrock, A.P., Jones, E.A., Forster, D., Nagaraju, R.T., Evangelou, C., Kamarashev, J., Green, M., Fairclough, M., Barinaga-Rementeria Ramirez, I., He, S., Snaar-Jagalska, B.E., Hollywood, K., Dunn, W.B., Spaink, H.P., Smith, M.P., Lorigan, P., Claude, E., Williams, K.J., McMahon, A.W., Hurlstone, A. (2019) Enhanced fatty acid scavenging and glycerophospholipid metabolism accompanies melanocyte neoplasia progression in zebrafish. Cancer research. 79(9):2136-2151
Zhao, S., Chen, Y., Chen, F., Huang, D., Shi, H., Lo, L.J., Chen, J., Peng, J. (2019) Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10-Imp3-Imp4 complex to nucleolus. Nucleic acids research. 47(6):2996-3012
Li, Z., He, S., Look, A.T. (2019) The MCL1-specific inhibitor S63845 acts synergistically with venetoclax/ABT-199 to induce apoptosis in T-cell acute lymphoblastic leukemia cells. Leukemia. 33:262-266
Mansour, M.R., He, S., Li, Z., Lobbardi, R., Abraham, B.J., Hug, C., Rahman, S., Leon, T.E., Kuang, Y.Y., Zimmerman, M.W., Blonquist, T., Gjini, E., Gutierrez, A., Tang, Q., Garcia-Perez, L., Pike-Overzet, K., Anders, L., Berezovskaya, A., Zhou, Y., Zon, L.I., Neuberg, D., Fielding, A.K., Staal, F.J.T., Langenau, D.M., Sanda, T., Young, R.A., Look, A.T. (2018) JDP2: An oncogenic bZIP transcription factor in T cell acute lymphoblastic leukemia. The Journal of experimental medicine. 215(7):1929-1945
Zimmerman, M.W., Liu, Y., He, S., Durbin, A.D., Abraham, B.J., Easton, J., Shao, Y., Xu, B., Zhu, S., Zhang, X., Li, Z., Weichert-Leahey, N., Young, R.A., Zhang, J., Look, A.T. (2018) MYC Drives a Subset of High-Risk Pediatric Neuroblastomas and Is Activated through Mechanisms Including Enhancer Hijacking and Focal Enhancer Amplification. Cancer discovery. 8:320-335
Lobbardi, R., Pinder, J., Martinez-Pastor, B., Theodorou, M., Blackburn, J.S., Abraham, B.J., Namiki, Y., Mansour, M., Abdelfattah, N.S., Molodtsov, A., Alexe, G., Toiber, D., de Waard, M., Jain, E., Boukhali, M., Lion, M., Bhere, D., Shah, K., Gutierrez, A., Stegmaier, K., Silverman, L.B., Sadreyev, R.I., Asara, J.M., Oettinger, M.A., Haas, W., Look, A.T., Young, R.A., Mostoslavsky, R., Dellaire, G., Langenau, D.M. (2017) TOX regulates growth, DNA repair, and genomic instability in T-cell Acute Lymphoblastic Leukemia. Cancer discovery. 7(11):1336-1353
Zhu, S., Zhang, X., Weichert-Leahey, N., Dong, Z., Zhang, C., Lopez, G., Tao, T., He, S., Wood, A.C., Oldridge, D., Ung, C.Y., van Ree, J.H., Khan, A., Salazar, B.M., Lummertz da Rocha, E., Zimmerman, M.W., Guo, F., Cao, H., Hou, X., Weroha, S.J., Perez-Atayde, A.R., Neuberg, D.S., Meves, A., McNiven, M.A., van Deursen, J.M., Li, H., Maris, J.M., Look, A.T. (2017) LMO1 Synergizes with MYCN to Promote Neuroblastoma Initiation and Metastasis. Cancer Cell. 32(3):310-323.e5
Liu, X., Li, Y.S., Shinton, S.A., Rhodes, J., Tang, L., Feng, H., Jette, C.A., Look, A.T., Hayakawa, K., Hardy, R.R. (2017) Zebrafish B Cell Development without a Pre-B Cell Stage, Revealed by CD79 Fluorescence Reporter Transgenes. Journal of immunology (Baltimore, Md. : 1950). 199(5):1706-1715
Liau, W.S., Tan, S.H., Ngoc, P.C., Wang, C.Q., Tergaonkar, V., Feng, H., Gong, Z., Osato, M., Look, A.T., Sanda, T. (2017) Aberrant activation of the GIMAP enhancer by oncogenic transcription factors in T-cell acute lymphoblastic leukemia. Leukemia. 31(8):1798-1807
Tao, T., Sondalle, S.B., Shi, H., Zhu, S., Perez-Atayde, A.R., Peng, J., Baserga, S.J., Look, A.T. (2017) The pre-rRNA processing factor DEF is rate limiting for the pathogenesis of MYCN-driven neuroblastoma. Oncogene. 36(27):3852-3867
Zhang, X., Dong, Z., Zhang, C., Ung, C.Y., He, S., Tao, T., Oliveira, A.M., Meves, A., Ji, B., Look, A.T., Li, H., Neel, B.G., Zhu, S. (2017) Critical Role for GAB2 in Neuroblastoma Pathogenesis through the Promotion of SHP2/MYCN Cooperation. Cell Reports. 18:2932-2942
Ki, D.H., He, S., Rodig, S., Look, A.T. (2017) Overexpression of PDGFRA cooperates with loss of NF1 and p53 to accelerate the molecular pathogenesis of malignant peripheral nerve sheath tumors. Oncogene. 36(8):1058-1068
He, S., Jing, C.B., Look, A.T. (2017) Zebrafish models of leukemia. Methods in cell biology. 138:563-592
Anderson, N.M., Li, D., Peng, H.L., Laroche, F.J., Mansour, M.R., Gjini, E., Aioub, M., Helman, D.J., Roderick, J.E., Cheng, T., Harrold, I., Samaha, Y., Meng, L., Amsterdam, A., Neuberg, D.S., Denton, T.T., Sanda, T., Kelliher, M.A., Singh, A., Look, A.T., Feng, H. (2016) The TCA cycle transferase DLST is important for MYC-mediated leukemogenesis. Leukemia. 30(6):1365-74
He, S., Mansour, M.R., Zimmerman, M.W., Ki, D.H., Layden, H.M., Akahane, K., Gjini, E., de Groh, E.D., Perez-Atayde, A.R., Zhu, S., Epstein, J.A., Look, A.T. (2016) Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain. eLIFE. 5
Tang, Q., Moore, J.C., Ignatius, M.S., Tenente, I.M., Hayes, M.N., Garcia, E.G., Torres Yordán, N., Bourque, C., He, S., Blackburn, J.S., Look, A.T., Houvras, Y., Langenau, D.M. (2016) Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish. Nature communications. 7:10358
Durbin, A.D., Ki, D.H., He, S., Look, A.T. (2016) Malignant Peripheral Nerve Sheath Tumors. Advances in experimental medicine and biology. 916:495-530
Morrison, M.A., Zimmerman, M.W., Look, A.T., Stewart, R.A. (2016) Studying the peripheral sympathetic nervous system and neuroblastoma in zebrafish. Methods in cell biology. 134:97-138
Tulotta, C., He, S., Chen, L., Groenewoud, A., van der Ent, W., Meijer, A.H., Spaink, H.P., Snaar-Jagalska, B.E. (2016) Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model. Methods in molecular biology (Clifton, N.J.). 1451:155-69
Zhu, S., Look, A. T. (2016) Neuroblastoma and Its Zebrafish Model. Advances in experimental medicine and biology. 916:451-78
Breyer, M.D., Look, A.T., Cifra, A. (2015) From bench to patient: model systems in drug discovery. Disease models & mechanisms. 8:1171-4
Deveau, A.P., Forrester, A.M., Coombs, A.J., Wagner, G.S., Grabher, C., Chute, I.C., Léger, D., Mingay, M., Alexe, G., Rajan, V., Liwski, R., Hirst, M., Steigmaier, K., Lewis, S.M., Look, A.T., Berman, J.N. (2015) Epigenetic therapy restores normal hematopoiesis in a zebrafish model of NUP98-HOXA9-induced myeloid disease. Leukemia. 29(10):2086-97
Jia, X.E., Ma, K., Xu, T., Gao, L., Wu, S., Fu, C., Zhang, W., Wang, Z., Liu, K., Dong, M., Jing, C., Ren, C., Dong, Z., Chen, Y., Jin, Y., Huang, Q., Chang, X., Deng, M., Li, L., Luo, L., Zhu, J., Dang, Y., Chang, H.C., Zon, L.I., Zhou, Y., Chen, S., Pan, W. (2015) Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction. Cell Research. 25(8):946-62
Gao, L., Li, D., Ma, K., Zhang, W., Xu, T., Fu, C., Jing, C., Jia, X., Wu, S., Sun, X., Dong, M., Deng, M., Chen, Y., Zhu, W., Peng, J., Wan, F., Zhou, Y., Zon, L.I., Pan, W. (2015) TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis. PLoS Genetics. 11:e1005346
Gjini, E., Mansour, M.R., Sander, J.D., Moritz, N., Nguyen, A.T., Kesarsing, M., Gans, E., He, S., Chen, S., Ko, M., Kuang, Y.Y., Yang, S., Zhou, Y., Rodig, S., Zon, L.I., Joung, J.K., Rao, A., Look, A.T. (2015) A Zebrafish Model of Myelodysplastic Syndrome Produced Through tet2 Genomic Editing. Molecular and cellular biology. 35(5):789-804
Gong, L., Gong, H., Pan, X., Chang, C., Ou, Z., Ye, S., Yin, L., Yang, L., Tao, T., Zhang, Z., Liu, C., Lane, D.P., Peng, J., Chen, J. (2015) p53 isoform Δ113p53/Δ133p53 promotes DNA double-strand break repair to protect cell from death and senescence in response to DNA damage. Cell Research. 25(3):351-69
Ghotra, V.P., He, S., van der Horst, G., Nijhoff, S., de Bont, H., Lekkerkerker, A., Janssen, R., Jenster, G., van Leenders, G.J., Hoogland, A.M., Verhoef, E.I., Baranski, Z., Xiong, J., van de Water, B., van der Pluijm, G., Snaar-Jagalska, E., Danen, E. (2015) SYK is a candidate kinase target for the treatment of advanced prostate cancer. Cancer research. 75(1):230-40
Shi, H., Tao, T., Huang, D., Ou, Z., Chen, J., Peng, J. (2015) A naturally occurring 4-bp deletion in the intron 4 of p53 creates a spectrum of novel p53 isoforms with anti-apoptosis function. Nucleic acids research. 43(2):1035-43
Narla, A., Payne, E.M., Abayasekara, N., Hurst, S.N., Raiser, D.M., Look, A.T., Berliner, N., Ebert, B.L., Khanna-Gupta, A. (2014) L-Leucine improves the anaemia in models of Diamond Blackfan anaemia and the 5q- syndrome in a TP53-independent way. British journal of haematology. 167(4):524-8
Gutierrez, A., Feng, H., Stevenson, K., Neuberg, D.S., Calzada, O., Zhou, Y., Langenau, D.M., Look, A.T. (2014) Loss of function tp53 mutations do not accelerate the onset of myc-induced T-cell acute lymphoblastic leukaemia in the zebrafish. British journal of haematology. 166(1):84-90
Truong, H.H., Xiong, J., Ghotra, V.P., Nirmala, E., Haazen, L., Le Dévédec, S.E., Balcioğlu, H.E., He, S., Snaar-Jagalska, B.E., Vreugdenhil, E., Meerman, J.H., van de Water, B., Danen, E.H. (2014) β1 integrin inhibition elicits a prometastatic switch through the TGFβ-miR-200-ZEB network in E-cadherin-positive triple-negative breast cancer.. Science signaling. 7(312):ra15
Gutierrez, A., Pan, L., Groen, R.W., Baleydier, F., Kentsis, A., Marineau, J., Grebliunaite, R., Kozakewich, E., Reed, C., Pflumio, F., Poglio, S., Uzan, B., Clemons, P., Verplank, L., An, F., Burbank, J., Norton, S., Tolliday, N., Steen, H., Weng, A.P., Yuan, H., Bradner, J.E., Mitsiades, C., Look, A.T., and Aster, J.C. (2014) Phenothiazines induce PP2A-mediated apoptosis in T cell acute lymphoblastic leukemia. J. Clin. Invest.. 124(2):644-655
Drabsch, Y., He, S., Zhang, L., Snaar-Jagalska, B.E., and Ten Dijke, P. (2013) Transforming growth factor beta signalling controls human breast cancer metastasis in a zebrafish xenograft model. Breast cancer research : BCR. 15(6):R106
Wang, S., Yin, J., Chen, D., Nie, F., Song, X., Fei, C., Miao, H., Jing, C., Ma, W., Wang, L., Xie, S., Li, C., Zeng, R., Pan, W., Hao, X., and Li, L. (2013) Small-molecule modulation of Wnt signaling via modulating the Axin-LRP5/6 interaction. Nature Chemical Biology. 9(9):579-85
Zuo, P., Fu, Z., Tao, T., Ye, F., Chen, L., Wang, X., Lu, W., and Xie, X. (2013) The expression of glia maturation factors and the effect of glia maturation factor-gamma on angiogenic sprouting in zebrafish. Experimental cell research. 319(5):707-717
Shin, J., Padmanabhan, A., de Groh, E.D., Lee, J.S., Haidar, S., Dahlberg, S., Guo, F., He, S., Wolman, M.A., Granato, M., Lawson, N.D., Wolfe, S.A., Kim, S.H., Solnica-Krezel, L., Kanki, J.P., Ligon, K.L., Epstein, J.A., and Look, A.T. (2012) Zebrafish neurofibromatosis type 1 genes have redundant functions in tumorigenesis and embryonic development. Disease models & mechanisms. 5(6):881-894
Payne, E., Virgilio, M., Narla, A., Sun, H., Levine, M., Paw, B.H., Berliner, N., Look, A.T., Ebert, B., and Khanna-Gupta, A. (2012) L-leucine improves the anemia and developmental defects associated with Diamond-Blackfan anemia and del(5q) MDS by activating the mTOR pathway. Blood. 120(11):2214-2224
Blackburn, J.S., Liu, S., Raiser, D.M., Martinez, S.A., Feng, H., Meeker, N.D., Gentry, J., Neuberg, D., Look, A.T., Ramaswamy, S., Bernards, A., Trede, N.S., and Langenau, D.M. (2012) Notch signaling expands a pre-malignant pool of T-cell acute lymphoblastic leukemia clones without affecting leukemia-propagating cell frequency. Leukemia. 26(9):2069-2078
He, S., Lamers, G.E., Beenakker, J.W., Cui, C., Ghotra, V.P., Danen, E.H., Meijer, A.H., Spaink, H.P., and Snaar-Jagalska, B.E. (2012) Neutrophil-mediated experimental metastasis is enhanced by VEGFR inhibition in a zebrafish xenograft model. The Journal of pathology. 227(4):431-445
Zhu, S., Lee, J.S., Guo, F., Shin, J., Perez-Atayde, A.R., Kutok, J.L., Rodig, S.J., Neuberg, D.S., Helman, D., Feng, H., Stewart, R.A., Wang, W., George, R.E., Kanki, J.P., and Look, A.T. (2012) Activated ALK Collaborates with MYCN in Neuroblastoma Pathogenesis. Cancer Cell. 21(3):362-373
Ghotra, V.P., He, S., de Bont, H., van der Ent, W., Spaink, H.P., van de Water, B., Snaar-Jagalska, B.E., and Danen, E.H. (2012) Automated whole animal bio-imaging assay for human cancer dissemination. PLoS One. 7(2):e31281
Sorrells, S., Carbonneau, S., Harrington, E., Chen, A.T., Hast, B., Milash, B., Pyati, U., Major, M.B., Zhou, Y., Zon, L.I., Stewart, R.A., Look, A.T., and Jette, C. (2012) Ccdc94 Protects Cells from Ionizing Radiation by Inhibiting the Expression of p53. PLoS Genetics. 8(8):e1002922
Rudner, L.A., Brown, K.H., Dobrinski, K.P., Bradley, D.F., Garcia, M.I., Smith, A.C., Downie, J.M., Meeker, N.D., Look, A.T., Downing, J.R., Gutierrez, A., Mullighan, C.G., Schiffman, J.D., Lee, C., Trede, N.S., and Frazer, J.K. (2011) Shared acquired genomic changes in zebrafish and human T-ALL. Oncogene. 30(41):4289-96
Forrester, A.M., Grabher, C., McBride, E.R., Boyd, E.R., Vigerstad, M.H., Edgar, A., Kai, F.B., Da'as, S.I., Payne, E., Look, A.T., and Berman, J.N. (2011) NUP98-HOXA9-transgenic zebrafish develop a myeloproliferative neoplasm and provide new insight into mechanisms of myeloid leukaemogenesis. British journal of haematology. 155(2):167-81
Zhu, C., Smith, T., McNulty, J., Rayla, A.L., Lakshmanan, A., Siekmann, A.F., Buffardi, M., Meng, X., Shin, J., Padmanabhan, A., Cifuentes, D., Giraldez, A.J., Look, A.T., Epstein, J.A., Lawson, N.D., and Wolfe, S.A. (2011) Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development (Cambridge, England). 138(20):4555-4564
Gutierrez, A., Snyder, E.L., Marino-Enriquez, A., Zhang, Y.X., Sioletic, S., Kozakewich, E., Grebliunaite, R., Ou, W.B., Sicinska, E., Raut, C.P., Demetri, G.D., Perez-Atayde, A.R., Wagner, A.J., Fletcher, J.A., Fletcher, C.D., and Look, A.T. (2011) Aberrant AKT activation drives well-differentiated liposarcoma. Proceedings of the National Academy of Sciences of the United States of America. 108(39):16386-91
Pyati, U.J., Gjini, E., Carbonneau, S., Lee, J.S., Guo, F., Jette, C.A., Kelsell, D.P., and Look, A.T. (2011) p63 Mediates an Apoptotic Response to Pharmacological and Disease-Related ER Stress in the Developing Epidermis. Developmental Cell. 21(3):492-505
He, S., Krens, S.G., Zhan, H., Gong, Z., Hogendoorn, P.C., Spaink, H.P., and Snaar-Jagalska, B.E. (2011) A DeltaRaf1-ER-inducible oncogenic zebrafish liver cell model identifies hepatocellular carcinoma signatures. The Journal of pathology. 225(1):19-28
Gutierrez, A., Grebliunaite, R., Feng, H., Kozakewich, E., Zhu, S., Guo, F., Payne, E., Mansour, M., Dahlberg, S.E., Neuberg, D.S., Hertog, J.D., Prochownik, E.V., Testa, J.R., Harris, M., Kanki, J.P., and Look, A.T. (2011) Pten mediates Myc oncogene dependence in a conditional zebrafish model of T cell acute lymphoblastic leukemia. The Journal of experimental medicine. 208(8):1595-603
Payne, E.M., Bolli, N., Rhodes, J., Abdel-Wahab, O.I., Levine, R., Hedvat, C.V., Stone, R., Khanna-Gupta, A., Sun, H., Kanki, J.P., Gazda, H.T., Beggs, A.H., Cotter, F.E., and Look, A.T. (2011) Ddx18 is essential for cell cycle progression in zebrafish hematopoietic cells and is mutated in human acute myeloid leukemia. Blood. 118(4):903-15
Bolli, N., Payne, E.M., Rhodes, J., Gjini, E., Johnston, A.B., Guo, F., Lee, J.S., Stewart, R.A., Kanki, J.P., Chen, A.T., Zhou, Y., Zon, L.I., and Look, A.T. (2011) cpsf1 is required for definitive HSC survival in zebrafish. Blood. 117(15):3996-4007
Grabher, C., Payne, E.M., Johnston, A.B., Bolli, N., Lechman, E., Dick, J.E., Kanki, J.P., and Look, A.T. (2011) Zebrafish microRNA-126 determines hematopoietic cell fate through c-Myb. Leukemia. 25(3):506-514
Robinson, B.W., Germano, G., Song, Y., Abrams, J., Scott, M., Guariento, I., Tiso, N., Argenton, F., Basso, G., Rhodes, J., Kanki, J.P., Look, A.T., Balice-Gordon, R.J., and Felix, C.A. (2011) mll ortholog containing functional domains of human MLL is expressed throughout the zebrafish lifespan and in haematopoietic tissues. British journal of haematology. 152(3):307-321
Etchin, J., Kanki, J.P., and Look, A.T. (2011) Zebrafish as a model for the study of human cancer. Methods in cell biology. 105:309-337
Lee, J.S., Padmanabhan, A., Shin, J., Zhu, S., Guo, F., Kanki, J.P., Epstein, J.A., and Look, A.T. (2010) Oligodendrocyte progenitor cell numbers and migration are regulated by the zebrafish orthologs of the NF1 tumor suppressor gene. Human molecular genetics. 19(23):4643-4653
Feng, H., Stachura, D.L., White, R.M., Gutierrez, A., Zhang, L., Sanda, T., Jette, C.A., Testa, J.R., Neuberg, D.S., Langenau, D.M., Kutok, J.L., Zon, L.I., Traver, D., Fleming, M.D., Kanki, J.P., and Look, A.T. (2010) T-lymphoblastic lymphoma cells express high levels of BCL2, S1P1, and ICAM1, leading to a blockade of tumor cell intravasation. Cancer Cell. 18(4):353-366
Kabli, S., He, S., Spaink, H.P., Hurlstone, A., Jagalska, E.S., De Groot, H.J., and Alia, A. (2010) In vivo magnetic resonance imaging to detect malignant melanoma in adult zebrafish. Zebrafish. 7(2):143-148
Stewart, R.A., Sanda, T., Widlund, H.R., Zhu, S., Swanson, K.D., Hurley, A.D., Bentires-Alj, M., Fisher, D.E., Kontaridis, M.I., Look, A.T., and Neel, B.G. (2010) Phosphatase-Dependent and -Independent Functions of Shp2 in Neural Crest Cells Underlie LEOPARD Syndrome Pathogenesis. Developmental Cell. 18(5):750-762
Bolli, N., Payne, E.M., Grabher, C., Lee, J.S., Johnston, A.B., Falini, B., Kanki, J.P., and Look, A.T. (2010) Expression of the cytoplasmic NPM1 mutant (NPMc+) causes the expansion of hematopoietic cells in zebrafish. Blood. 115(16):3329-3340
Stewart, R.A., Lee, J.S., Lachnit, M., Look, A.T., Kanki, J.P., and Henion, P.D. (2010) Chapter 5 - Studying peripheral sympathetic nervous system development and neuroblastoma in zebrafish. Methods in cell biology. 100:127-152
Padmanabhan, A., Lee, J.S., Ismat, F.A., Lu, M.M., Lawson, N.D., Kanki, J.P., Look, A.T., and Epstein, J.A. (2009) Cardiac and vascular functions of the zebrafish orthologues of the type I neurofibromatosis gene NFI. Proceedings of the National Academy of Sciences of the United States of America. 106(52):22305-22310
Rhodes, J., Amsterdam, A., Sanda, T., Moreau, L.A., McKenna, K., Heinrichs, S., Ganem, N.J., Ho, K.W., Neuberg, D.S., Johnston, A., Ahn, Y., Kutok, J.L., Hromas, R., Wray, J., Lee, C., Murphy, C., Radke, I., Downing, J.R., Fleming, M.D., Macconaill, L.E., Amatruda, J.F., Gutierrez, A., Galinsky, I., Stone, R.M., Ross, E.A., Pellman, D.S., Kanki, J.P., and Look, A.T. (2009) Emi1 Maintains Genomic Integrity during Zebrafish Embryogenesis and Cooperates with p53 in Tumor Suppression. Molecular and cellular biology. 29(21):5911-5922
Dodd, M.E., Hatzold, J., Mathias, J.R., Walters, K.B., Bennin, D.A., Rhodes, J., Kanki, J.P., Look, A.T., Hammerschmidt, M., and Huttenlocher, A. (2009) The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish. Development (Cambridge, England). 136(15):2591-2600
Payne, E., and Look, T. (2009) Zebrafish modelling of leukaemias. British journal of haematology. 146(3):247-256
Niethammer, P., Grabher, C., Look, A.T., and Mitchison, T.J. (2009) A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish. Nature. 459(7249):996-999
Chen, C.C., Kennedy, R.D., Sidi, S., Look, A.T., and D'Andrea, A.D. (2009) CHK1 inhibition as a strategy for targeting fanconi anemia (FA) DNA repair pathway deficient tumors. Molecular Cancer. 8:24
Freeman, J.L., Ceol, C., Feng, H., Langenau, D.M., Belair, C., Stern, H.M., Song, A., Paw, B.H., Look, A.T., Zhou, Y., Zon, L.I., and Lee, C. (2009) Construction and application of a zebrafish array comparative genomic hybridization platform. Genes, chromosomes & cancer. 48(2):155-170
Walters, K.B., Dodd, M.E., Mathias, J.R., Gallagher, A.J., Bennin, D.A., Rhodes, J., Kanki, J.P., Look, A.T., Grinblat, Y., and Huttenlocher, A. (2009) Muscle degeneration and leukocyte infiltration caused by mutation of zebrafish fad24. Developmental dynamics : an official publication of the American Association of Anatomists. 238(1):86-99
Sessa, A.K., White, R., Houvras, Y., Burke, C., Pugach, E., Baker, B., Gilbert, R., Look, A.T., and Zon, L.I. (2008) The effect of a depth gradient on the mating behavior, oviposition site preference, and embryo production in the zebrafish, Danio rerio. Zebrafish. 5(4):335-339
Zhang, Y., Bai, X.T., Zhu, K.Y., Jin, Y., Deng, M., Le, H.Y., Fu, Y.F., Chen, Y., Zhu, J., Look, A.T., Kanki, J., Chen, Z., Chen, S.J., and Liu, T.X. (2008) In Vivo Interstitial Migration of Primitive Macrophages Mediated by JNK-Matrix Metalloproteinase 13 Signaling in Response to Acute Injury. Journal of immunology (Baltimore, Md. : 1950). 181(3):2155-2164
Krens, S.F., He, S., Lamers, G.E., Meijer, A.H., Bakkers, J., Schmidt, T., Spaink, H.P., and Snaar-Jagalska, B.E. (2008) Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation. Developmental Biology. 319(2):370-383
Langenau, D.M., Keefe, M.D., Storer, N.Y., Jette, C.A., Smith, A.C., Ceol, C.J., Bourque, C., Look, A.T., and Zon, L.I. (2008) Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish. Oncogene. 27(30):4242-4248
Jette, C.A., Flanagan, A.M., Ryan, J., Pyati, U.J., Carbonneau, S., Stewart, R.A., Langenau, D.M., Look, A.T., and Letai, A. (2008) BIM and other BCL-2 family proteins exhibit cross-species conservation of function between zebrafish and mammals. Cell death and differentiation. 15(6):1063-1072
Sidi, S., Sanda, T., Kennedy, R.D., Hagen, A.T., Jette, C.A., Hoffmans, R., Pascual, J., Imamura, S., Kishi, S., Amatruda, J.F., Kanki, J.P., Green, D.R., D'Andrea, A.A., and Look, A.T. (2008) Chk1 Suppresses a Caspase-2 Apoptotic Response to DNA Damage that Bypasses p53, Bcl-2, and Caspase-3. Cell. 133(5):864-877
Krens, S.F., Corredor-Adamez, M., He, S., Snaar-Jagalska, B.E., and Spaink, H.P. (2008) ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis. BMC Genomics. 9:196
Mathias, J.R., Dodd, M.E., Walters, K.B., Rhodes, J., Kanki, J.P., Look, A.T., and Huttenlocher, A. (2007) Live imaging of chronic inflammation caused by mutation of zebrafish Hai1. Journal of Cell Science. 120(19):3372-3383
Smolen, G.A., Schott, B.J., Stewart, R.A., Diederichs, S., Muir, B., Provencher, H.L., Look, A.T., Sgroi, D.C., Peterson, R.T., and Haber, D.A. (2007) A Rap GTPase interactor, RADIL, mediates migration of neural crest precursors. Genes & Development. 21(17):2131-2136
Feng, H., Langenau, D.M., Madge, J.A., Quinkertz, A., Gutierrez, A., Neuberg, D.S., Kanki, J.P., and Look, A.T. (2007) Heat-shock induction of T-cell lymphoma/leukaemia in conditional Cre/lox-regulated transgenic zebrafish. British journal of haematology. 138(2):169-175
Pyati, U.J., Look, A.T., and Hammerschmidt, M. (2007) Zebrafish as a powerful vertebrate model system for in vivo studies of cell death. Seminars in Cancer Biology. 17(2):154-165
Chen, J., Jette, C., Kanki, J.P., Aster, J.C., Look, A.T., and Griffin, J.D. (2007) NOTCH1-induced T-cell leukemia in transgenic zebrafish. Leukemia. 21(3):462-471
Liu, T.X., Rhodes, J., Deng, M., Hsu, K., Radomska, H.S., Kanki, J.P., Tenen, D.G., and Look, A.T. (2007) Dominant-interfering C/EBPalpha stimulates primitive erythropoiesis in zebrafish. Experimental hematology. 35(2):230-239
He, S., Salas-Vidal, E., Rueb, S., Krens, S.F.G., Meijer, A.H., Snaar-Jagalska, B.E., and Spaink, H.P. (2006) Genetic and Transcriptome Characterization of Model Zebrafish Cell Lines. Zebrafish. 3(4):441-453
Mathias, J.R., Perrin, B.J., Liu, T.X., Kanki, J., Look, A.T., and Huttenlocher, A. (2006) Resolution of inflammation by retrograde chemotaxis of neutrophils in transgenic zebrafish. Journal of Leukocyte Biology. 80(6):1281-1288
Krens, S.F., He, S., Spaink, H.P., and Snaar-Jagalska, B.E. (2006) Characterization and expression patterns of the MAPK family in zebrafish. Gene expression patterns : GEP. 6(8):1019-1026
Grabher, C., von Boehmer, H., and Look, A.T. (2006) Notch 1 activation in the molecular pathogenesis of T-cell acute lymphoblastic leukaemia. Nature reviews. Cancer. 6(5):347-359
Stewart, R.A., Arduini, B.L., Berghmans, S., George, R.E., Kanki, J.P., Henion, P.D., and Look, A.T. (2006) Zebrafish foxd3 is selectively required for neural crest specification, migration and survival. Developmental Biology. 292(1):174-188
Grabher, C., and Look, A.T. (2006) Fishing for cancer models. Nat. Biotechnol.. 24(1):45-46
Sidi, S., and Look, A.T. (2005) Small molecules thwart crash and burn. Nature Chemical Biology. 1(7):351-353
Berman, J.N., Kanki, J.P., and Look, A.T. (2005) Zebrafish as a model for myelopoiesis during embryogenesis. Experimental hematology. 33(9):997-1006
Berghmans, S., Jette, C., Langenau, D., Hsu, K., Stewart, R., Look, T., and Kanki, J.P. (2005) Making waves in cancer research: new models in the zebrafish. Biotechniques. 39(2):227-237
Langenau, D.M., Feng, H., Berghmans, S., Kanki, J.P., Kutok, J.L., and Look, A.T. (2005) Cre/lox-regulated transgenic zebrafish model with conditional myc-induced T cell acute lymphoblastic leukemia. Proceedings of the National Academy of Sciences of the United States of America. 102(17):6068-6073
Langenau, D.M., Jette, C., Berghmans, S., Palomero, T., Kanki, J.P., Kutok, J.L., and Look, A.T. (2005) Suppression of apoptosis by bcl-2-overexpression in lymphoid cells of transgenic zebrafish. Blood. 105(8):3278-3285
Patton, E.E., Widlund, H.R., Kutok, J.L., Kopani, K.R., Amatruda, J.F., Murphey, R.D., Berghmans, S., Mayhall, E.A., Traver, D., Fletcher, C.D., Aster, J.C., Granter, S.R., Look, A.T., Lee, C., Fisher, D.E., and Zon, L.I. (2005) BRAF Mutations Are Sufficient to Promote Nevi Formation and Cooperate with p53 in the Genesis of Melanoma. Current biology : CB. 15(3):249-254
Berghmans,, S., Murphey, R.D., Wienholds, E., Neuberg, D., Kutok, J.L., Fletcher, C.D., Morris, J.P., Liu, T.X., Schulte-Merker, S., Kanki, J.P., Plasterk, R., Zon, L.I., and Look, A.T. (2005) tp53 mutant zebrafish develop malignant peripheral nerve sheath tumors. Proceedings of the National Academy of Sciences of the United States of America. 102(2):407-412
Rhodes, J., Hagen, A., Hsu, K., Deng, M., Liu, T.X., Look, A.T., and Kanki, J.P. (2005) Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish. Developmental Cell. 8(1):97-108
Yang, H.W., Kutok, J.L., Lee, N.H., Piao, H.Y., Fletcher, C.D., Kanki, J.P., and Look, A.T. (2004) Targeted Expression of Human MYCN Selectively Causes Pancreatic Neuroendocrine Tumors in Transgenic Zebrafish. Cancer research. 64(20):7256-7262
Hsu, K., Traver, D., Kutok, J.L., Hagen, A., Liu, T.X., Paw, B.H., Rhodes, J., Berman, J., Zon, L.I., Kanki, J.P., and Look, A.T. (2004) The pu.1 promoter drives myeloid gene expression in zebrafish. Blood. 104(5):1291-1297
Traver, D., Winzeler, A., Stern, H.M., Mayhall, E.A., Langenau, D.M., Kutok, J.L., Look, A.T., and Zon, L.I. (2004) Effects of lethal irradiation in zebrafish and rescue by hematopoietic cell transplantation. Blood. 104(5):1298-1305
Langenau, D.M., Ferrando, A.A., Traver, D., Kutok, J.L., Hezel, J.P., Kanki, J.P., Zon, L.I., Look, A.T., and Trede, N.S. (2004) In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 101(19):7369-7374
Trede, N.S., Langenau, D.M., Traver, D., Look, A.T., and Zon, L.I. (2004) The use of zebrafish to understand immunity. Immunity. 20(4):367-379
Berghmans, S., Morris, J.P. 4th, Kanki, J.P., and Look, A.T. (2004) Zebrafish sperm cryopreservation. The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:645-659
Hsu, K., Look, A.T., and Kanki, J.P. (2004) Lessons from transgenic zebrafish expressing the green fluorescent protein (GFP) in the myeloid lineage. The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:333-347
Stewart, R.A., Look, A.T., Kanki, J.P., and Henion, P.D. (2004) Development of the peripheral sympathetic nervous system in zebrafish. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:237-260
Liu, T.X., Howlett, N.G., Deng, M., Langenau, D.M., Hsu, K., Rhodes, J., Kanki, J.P., D'Andrea, A.D., and Look, A.T. (2003) Knockdown of zebrafish fancd2 causes developmental abnormalities via p53-dependent apoptosis. Developmental Cell. 5(6):903-914
Berman, J., Hsu, K., and Look, A.T. (2003) Zebrafish as a model organism for blood diseases. British journal of haematology. 123(4):568-576
Morris IV, J.P., Berghmans, S., Zahrieh, D., Neuberg, D.S., Kanki, J.P., and Look, A.T. (2003) Zebrafish sperm cryopreservation with N,N-dimethylacetamide. Biotechniques. 35(5):956-958
Langenau, D.M., Traver, D., Ferrando, A.A., Kutok, J.L., Aster, J.C., Kanki, J.P., Lin, S., Prochownik, E., Trede, N.S., Zon, L.I., and Look, A.T. (2003) Myc-induced T cell leukemia in transgenic zebrafish. Science (New York, N.Y.). 299(5608):887-890
Langenau, D., Palomero, T., Kanki, J., Ferrando, A., Zhou, Y., Zon, L., and Look, A. (2002) Molecular cloning and developmental expression of Tlx (Hox11) genes in zebrafish (Danio rerio). Mechanisms of Development. 117(1-2):243-248
Liu, T.-X., Zhou, Y., Kanki, J.P., Deng, M., Rhodes, J., Yang, H.-W., Sheng, X.-M., Zon, L.I., and Look, A.T. (2002) Evolutionary conservation of zebrafish linkage group 14 with frequently deleted regions of human chromosome 5 in myeloid malignancies. Proceedings of the National Academy of Sciences of the United States of America. 99(9):6136-6141
Hsu, K., Rhodes, J., Liu, T.X., Kanki, J.P., Paw, B., Zon, L.I., and Look, A.T. (2001) Pu.1 is expressed by early granulocytic progenitors in the zebrafish. Blood. 98(11):63
Rhodes, J., Kanki, J.P., and Look, A.T. (2001) The role of Pu.1 in zebrafish hematopoiesis. Blood. 98(11):276
Song, H.D., Liu, T.X., Wu, X.Y., Shun, X.J., Zhang, Q.H., Chen, S.J., Zhou, Y., Chen, Z., Look, T.A., and Zon, L.I. (2001) Gene expression profiling in kidney of zebrafish's hematopoietic tissue. Blood. 98(11):4137
Bennett, C.M., Kanki, J.P., Rhodes, J., Liu, T.X., Paw, B.H., Kieran, M.W., Langenau, D.M., Delahaye-Brown, A., Zon, L.I., Fleming, M.D., and Look, A.T. (2001) Myelopoiesis in the zebrafish, Danio rerio. Blood. 98(3):643-651
Hsu, K., Kanki, J.P., and Look, A.T. (2001) Zebrafish myelopoiesis and blood cell development. Current opinion in hematology. 8(4):245-251
Bennett, C.M., Fleming, M.D., Kanki, J.P., Rhodes, J., Delahaye-Brown, A., Langenau, D.M., Liu, T.X., Paw, B.H., Zon, L.I., and Look, A.T. (2000) Genetic analysis of myelopoiesis the zebrafish. Blood. 96(11):4177
Liu, T.X., Zhou, Y., Kanki, J.P., Zon, L.I., and Look, A.T. (2000) Syntenic region shared by zebrafish and patients with 5q-syndrome. Blood. 96(11):380
Rhodes, J., Bennett, C.M., Kanki, J.P., Liu, T.X., Paw, B.H., Zon, L.I., and Look, A.T. (2000) Molecular analysis of zebrafish myelopoiesis. Blood. 96(11):3483