ZFIN Lab: Knapik Lab

ZFIN ID: ZDB-LAB-000316-1

Knapik Lab

PI/Director:	Knapik, Ela W.
Contact Person:	Ritter, Dylan J.
Email:	dylan.j.ritter@Vanderbilt.Edu
URL:
Address:	Division of Genetic Medicine, Department of Medicine, Vanderbilt University Medical Center, Department of Cell and Developmental Biology, Vanderbilt University 1165 Light Hall 2215 Garland Av. Nashville, TN 37232-0275
Country:	United States
Phone:	(615) 322-7559
Fax:	(615) 322-7549
Line Designation:	vmc

GENOMIC FEATURES ORIGINATING FROM THIS LAB

Show all 5 genomic features

STATEMENT OF RESEARCH INTERESTS

Our laboratory is studying molecular and cellular mechanisms governing neural crest specification and differentiation.

LAB MEMBERS

Choudhary, Dharmendra Post-Doc	Whitener, Amy Post-Doc	Luderman, Lauryn N. Graduate Student
Michaels, Mackenezie Graduate Student	Nagai, Taylor Graduate Student	Ritter, Dylan J. Graduate Student
Goodrich, Amanda L. Research Staff	Guthrie, Cory Lovel Research Staff

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Ritter, D.J., Choudhary, D., Unlu, G., Knapik, E.W. (2023) Rgp1 contributes to craniofacial cartilage development and Rab8a-mediated collagen II secretion. Frontiers in endocrinology. 14:11204201120420

Wareham, L.K., Whitener, A.E., Wu, H.J., Wu, S.Y., Mchaourab, H.S., Mortlock, D.P., Kuchtey, R.W., Kuchtey, J. (2022) Adamts10 controls transforming growth factor β family signaling that contributes to retinal ganglion cell development. Frontiers in molecular biosciences. 9:989851

Luderman, L.N., Michaels, M.T., Levic, D.S., Knapik, E.W. (2022) Zebrafish Erc1b mediates motor innervation and organization of craniofacial muscles in control of jaw movement. Developmental Dynamics : an official publication of the American Association of Anatomists. 252(1):104-123

Bayraktar, E.C., La, K., Karpman, K., Unlu, G., Ozerdem, C., Ritter, D.J., Alwaseem, H., Molina, H., Hoffmann, H.H., Millner, A., Atilla-Gokcumen, G.E., Gamazon, E.R., Rushing, A.R., Knapik, E.W., Basu, S., Birsoy, K. (2020) Metabolic coessentiality mapping identifies C12orf49 as a regulator of SREBP processing and cholesterol metabolism. Nature metabolism. 2:487-498

Unlu, G., Qi, X., Gamazon, E.R., Melville, D.B., Patel, N., Rushing, A.R., Hashem, M., Al-Faifi, A., Chen, R., Li, B., Cox, N.J., Alkuraya, F.S., Knapik, E.W. (2020) Phenome-based approach identifies RIC1-linked Mendelian syndrome through zebrafish models, biobank associations and clinical studies. Nature medicine. 26:98-109

Unlu, G., Gamazon, E.R., Qi, X., Levic, D.S., Bastarache, L., Denny, J.C., Roden, D.M., Mayzus, I., Breyer, M., Zhong, X., Konkashbaev, A.I., Rzhetsky, A., Knapik, E.W., Cox, N.J. (2019) GRIK5 Genetically Regulated Expression Associated with Eye and Vascular Phenomes: Discovery through Iteration among Biobanks, Electronic Health Records, and Zebrafish. American journal of human genetics. 104(3):503-519

Cox, N.J., Unlu, G., Bisnett, B.J., Meister, T.R., Condon, B., Luo, P.M., Smith, T.J., Hanna, M., Chhetri, A., Soderblom, E.J., Audhya, A., Knapik, E.W., Boyce, M. (2017) Dynamic glycosylation governs the vertebrate COPII protein trafficking pathway. Biochemistry. 57(1):91-107

Hockman, D., Burns, A., Schlosser, G., Gates, K.P., Jevans, B., Mongera, A., Fisher, S., Unlu, G., Knapik, E.W., Kaufman, C.K., Mosimann, C., Zon, L.I., Lancman, J.J., Dong, P.D., Lickert, H., Tucker, A.S., Baker, C.V. (2017) Evolution of the hypoxia-sensitive cells involved in amniote respiratory reflexes. eLIFE. 6

Luderman, L.N., Unlu, G., Knapik, E.W. (2017) Zebrafish Developmental Models of Skeletal Diseases. Current topics in developmental biology. 124:81-124

Zou, P., Wu, S.Y., Koteiche, H.A., Mishra, S., Levic, D.S., Knapik, E., Chen, W., Mchaourab, H.S. (2015) A conserved role of αA-crystallin in the development of the zebrafish embryonic lens. Experimental Eye Research. 138:104-13

Levic, D.S., Minkel, J., Wang, W., Rybski, W.M., Melville, D.B., Knapik, E.W. (2015) Animal model of Sar1b deficiency presents lipid absorption deficits similar to Anderson disease. Journal of molecular medicine (Berlin, Germany). 93(2):165-176

Niu, X., Hong, J., Zheng, X., Melville, D.B., Knapik, E.W., Meng, A., Peng, J. (2014) The Nuclear Pore Complex Function of Sec13 Protein Is Required for Cell Survival during Retinal Development. The Journal of biological chemistry. 289:11971-85

Vacaru, A.M., Unlu, G., Spitzner, M., Mione, M., Knapik, E.W., and Sadler, K.C. (2014) In vivo cell biology in zebrafish - providing insights into vertebrate development and disease. Journal of Cell Science. 127(Pt 3):485-495

Venkateswaran, A., Sekhar, K.R., Levic, D.S., Melville, D.B., Clark, T.A., Rybski, W.M., Walsh, A.J., Skala, M.C., Crooks, P.A., Knapik, E.W., and Freeman, M.L. (2014) The NADH Oxidase ENOX1, a Critical Mediator of Endothelial Cell Radiosensitization, Is Crucial for Vascular Development. Cancer research. 74(1):38-43

Lee, R.T., Knapik, E.W., Thiery, J.P., and Carney, T.J. (2013) An exclusively mesodermal origin of fin mesenchyme demonstrates that zebrafish trunk neural crest does not generate ectomesenchyme. Development (Cambridge, England). 140(14):2923-2932

Müller, I.I., Melville, D.B., Tanwar, V., Rybski, W.M., Mukherjee, A., Shoemaker, B.M., Wang, W.D., Schoenhard, J.A., Roden, D.M., Darbar, D., Knapik, E.W., and Hatzopoulos, A.K. (2013) Functional modeling in zebrafish demonstrates that the atrial-fibrillation-associated gene GREM2 regulates cardiac laterality, cardiomyocyte differentiation and atrial rhythm. Disease models & mechanisms. 6(2):332-341

Wang, W.D., Melville, D.B., Montero-Balaguer, M., Hatzopoulos, A.K., and Knapik, E.W. (2011) Tfap2a and Foxd3 regulate early steps in the development of the neural crest progenitor population. Developmental Biology. 360(1):173-85

Melville, D.B., Montero-Balaguer, M., Levic, D.S., Bradley, K., Smith, J.R., Hatzopoulos, A.K., and Knapik, E.W. (2011) The feelgood mutation in zebrafish dysregulates COPII-dependent secretion of select extracellular matrix proteins in skeletal morphogenesis. Disease models & mechanisms. 4(6):763-76

Eames, B.F., Yan, Y.L., Swartz, M.E., Levic, D.S., Knapik, E.W., Postlethwait, J.H., and Kimmel, C.B. (2011) Mutations in fam20b and xylt1 Reveal That Cartilage Matrix Controls Timing of Endochondral Ossification by Inhibiting Chondrocyte Maturation. PLoS Genetics. 7(8):e1002246

Bradley, K.M., Breyer, J.P., Melville, D.B., Broman, K.W., Knapik, E.W., and Smith, J.R. (2011) An SNP-Based Linkage Map for Zebrafish Reveals Sex Determination Loci. G3 (Bethesda). 1(1):3-9

Liu, D., Wang, W.D., Melville, D.B., Cha, Y.I., Yin, Z., Issaeva, N., Knapik, E.W., and Yarbrough, W.G. (2011) Tumor suppressor Lzap regulates cell cycle progression, doming, and zebrafish epiboly. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(6):1613-1625

Melville, D.B., and Knapik, E.W. (2011) Traffic jams in fish bones: ER-to-Golgi protein transport during zebrafish development. Cell adhesion & migration. 5(2):114-118

Sarmah, S., Barrallo-Gimeno, A., Melville, D.B., Topczewski, J., Solnica-Krezel, L., and Knapik, E.W. (2010) Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis. PLoS One. 5(4):e10367

Kucenas, S., Wang, W.D., Knapik, E.W., and Appel, B. (2009) A selective glial barrier at motor axon exit points prevents oligodendrocyte migration from the spinal cord. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(48):15187-15194

Granero-Moltó, F., Sarmah, S., O'Rear, L., Spagnoli, A., Abrahamson, D., Saus, J., Hudson, B.G., and Knapik, E.W. (2008) Goodpasture antigen-binding protein and its spliced variant, ceramide transfer protein, have different functions in the modulation of apoptosis during zebrafish development. The Journal of biological chemistry. 283(29):20495-20504

Bradley, K.M., Elmore, J.B., Breyer, J.P., Yaspan, B.L., Jessen, J.R., Knapik, E.W., and Smith, J.R. (2007) A major zebrafish polymorphism resource for genetic mapping. Genome biology. 8(4):R55

Montero-Balaguer, M., Lang, M.R., Sachdev, S.W., Knappmeyer, C., Stewart, R.A., De La Guardia, A., Hatzopoulos, A.K., and Knapik, E.W. (2006) The mother superior mutation ablates foxd3 activity in neural crest progenitor cells and depletes neural crest derivatives in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(12):3199-3212

Lang, M.R., Lapierre, L.A., Frotscher, M., Goldenring, J.R., and Knapik, E.W. (2006) Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturation. Nature Genetics. 38(10):1198-1203

Muller, I.I., Knapik, E.W., and Hatzopoulos, A.K. (2006) Expression of the protein related to Dan and Cerberus gene—prdc—During eye, pharyngeal arch, somite, and swim bladder development in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(10):2881-2888

Barrallo Gimeno, A., Holzschuh, J., Driever, W., and Knapik, E.W. (2004) Neural crest survival and differentiation in zebrafish depends on mont blanc/tfap2a gene function. Development (Cambridge, England). 131(7):1463-1477

Holzschuh, J., Barrallo Gimeno, A., Ettl, A.K., Durr, K., Knapik, E.W., and Driever, W. (2003) Noradrenergic neurons in the zebrafish hindbrain are induced by retinoic acid and require tfap2a for expression of the neurotransmitter phenotype. Development (Cambridge, England). 130(23):5741-5754

Dethleffsen, K., Heinrich, G., Lauth, M., Knapik, E.W., and Meyer, M. (2003) Insert-containing neurotrophins in teleost fish and their relationship to nerve growth factor. Molecular and cellular neurosciences. 24(2):380-394

Sachdev, S.W. and Knapik, E.W. (2001) Positional cloning and characterization of mother superior, a mutation affecting the layout of the pharyngeal skeleton. Molecular biology of the cell. (Suppl.) 12:2020

Sachdev, S.W., Dietz, U.H., Oshima, Y., Lang, M.R., Knapik, E.W., Hiraki, Y., and Shukunami, C. (2001) Sequence analysis of zebrafish chondromodulin-1 and expression profile in the notochord and chondrogenic regions during cartilage morphogenesis. Mechanisms of Development. 105(1-2):157-162

Knapik, E.W. (2000) ENU mutagenesis in zebrafish - from genes to complex diseases. Mammalian genome : official journal of the International Mammalian Genome Society. 11(7):511-519

Chevrette, M., Joly, L., Tellis, P., Knapik, E.W., Miles, J., Fishman, M., and Ekker, M. (2000) Characterization of a zebrafish/mouse somatic cell hybrid panel. Genomics. 64(1):119-26

Shimoda, N., Knapik, E.W., Ziniti, J., Sim, C., Yamada, E., Kaplan, S., Jackson, D., de Sauvage, F., Jacob, H., and Fishman, M.C. (1999) Zebrafish genetic map with 2000 microsatellite markers. Genomics. 58(3):219-232

Förnzler, D., Her, H., Knapik, E.W., Clark, M., Lehrach, H., Postlethwait, J.H., Zon, L.I., Beier, D.R. (1998) Gene mapping in zebrafish using single-strand conformation polymorphism analysis. Genomics. 51:216-222

Knapik, E.W., Goodman, A., Ekker, M., Chevrette, M., Delgado, J., Neuhauss, S., Shimoda, N., Driever, W., Fishman, M.C., and Jacob, H.J. (1998) A microsatellite genetic linkage map for zebrafish (Danio rerio). Nature Genetics. 18:338-343

Postlethwait, J.H, Yan, Y.-L., Gates, M.A., Horne, S., Amores, A., Brownlie, A., Donovan, A., Egan, E.S., Force, A., Gong, Z., Goutel, C., Fritz, A., Kelsh, R., Knapik, E., Liao, E., Paw, B., Ransom, D., Singer, A., Thomson, M., Abduljabbar, T.S., Yelick, P., Beier, D., Joly, J.-S., Larhammar, D., Rosa, F., Westerfield, M., Zon, L.I., Johnson, S.L., and Talbot, W.S. (1998) Vertebrate genome evolution and the zebrafish gene map. Nature Genetics. 18:345-349

Knapik, E.W., Goodman, A., Atkinson, O.S., Roberts, C.T., Shiozawa, M., Sim, C.U., Weksler-Zagen, S., Trolliet, M.R., Futrell, C., Innes, B.A., Koike, G., McLaughlin, M., Pierre, L., Simon, J.S., Vilallonga, E., Roy, M., Chiang, P.W., Fishman, M.C., Driever, W., and Jacob, H.J. (1996) A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms. Development (Cambridge, England). 123:451-460