ZFIN Image: McKaige et al., 2024, Fig. 4

Image

Figure Caption

Fig. 4 Analysis of autophagy markers reveals early defect in double mutants. (A) Double mutant fish show a significant reduction in autophagic flux activity at 6 dpf based on difference in LC3-II levels between NH4Cl treated and untreated zebrafish. Experiment was completed in triplicate. (B) Western blot at 6 dpf shows a significant accumulation of p62 in double mutants. Error bars represent SEM for three biological replicates. (C) At 3 mpf dnajb6b single mutants and double mutants show a significant reduction in p62 levels compared to wildtype. Error bars represent SEM for eight fish across 3 biological replicates. GAPDH was used as a loading control and P62 values are normalised to GAPDH. Datasets were analysed with a one-way ANOVA and a two-way Dunnett multiple comparisons test.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.