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Fig. 2 Double dnajb6 mutant zebrafish do not show signs of myopathy during early development. (A) Immunofluorescence staining of methyl cellulose treated dnajb6 mutants and their siblings with anti-myosin at 1 dpf. Larvae were incubated in methyl cellulose for 1 h prior to fixation. Maximum projection confocal images were taken laterally of the trunk above the egg yolk extension. (B) Images show examples of the phenotype scored. Quantification and analysis of the fibre damage phenotype showed no significant differences between genotypes (P > 0.05, binomial test). Analysis was completed with n ≥ 28 per genotype.