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Fig. 4 Tg(hsp70:E2A-PBX1-EGFP) adult fish exhibit abnormal myeloid cell expansion which resembles acute myeloid leukemia-like phenotypes. (A) May-Grunwald-Giemsa staining of peripheral blood (PB) cells (upper panels) and kidney marrow (KM) blood cells (lower panels) in 1-year-old wild-type (WT) (left panel) and Tg(hsp70:E2A-PBX1-EGFP) (right panel) adult fish after 3-month heat shock. Red arrows indicate myelocytes, black arrows indicate lymphocytes and green arrows indicate blasts. Original magnification ×400. Blood cell counts of PB and KM were calculated manually based on their morphology. (A’) Statistical analysis of cell counts in panel (A). The black asterisks indicate statistical difference (N=9, one-way ANOVA, mean ± standard error of the mean; *P<0.05, **P<0.01). (B) Kidneys from 1-year-old transgenic fish were enlarged to 1.36-fold in area compared with siblings after 3-month heat shock. (B’) Statistical analysis of the area of kidney in panel (B). The black asterisks indicate statistical difference (N=9, Student t tests, mean ± standard error of the mean; **P<0.01).