Fig 3

Fig 3 p53 activation by camptothecin treatment induces massive gene expression effects with a small contribution from miR-34a.

(A) Experimental design of the RNA sequencing experiment to analyze how miR-34a loss affects gene expression under normal and DNA damage treatment conditions. (B) Principal component analysis of gene expression values in all RNA sequencing samples. (C) Venn diagram for differentially expressed genes (DEGs) (fold-change ≥ 2 and FDR ≤ 0.05 as cut-offs) in the combined dataset (both genotypes), wild-type and miR-34a mutant subsets. (D) Hierarchical clustering of variance-stabilized transformed normalized counts (VST values) for all DEGs based on the treatment factor in full dataset. Treatment and genotype assignments are indicated at the top of the gene expression heatmap and the legend for the heatmap is shown to the right.