ZFIN Image: Wang et al., 2023, Fig. 2

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Figure Caption

Fig. 2 Human RCL1 protein stability is dependent on BMS1 protein level. (A) Western blot analysis of BMS1 and RCL1 protein levels in Hep3B and HUH7 cells after BMS1 knockdown by sh08 and sh07. shNC, negative control. TUBULIN: loading control. (B and C) qPCR analysis of the transcriptional levels of BMS1 and RCL1 in Hep3B (B) and HUH7 (C) cells after BMS1 knockdown by sh08 and sh07. The qPCR values were normalized against GAPDH and expressed as fold change of expression. (D and E) Western blot analysis of BMS1, RCL1 and RCL1-MYC protein levels after BMS1 knockdown and RCL1-MYC overexpression in Hela (D) or HepG2 (E) cells by doxycycline (Dox) treatment. (F and G) Western blot analysis of BMS1, BMS1-SFB, and RCL1 in monoclonal Hela cell lines (14# and 23#) with or without Dox treatment. The intensity ratio of RCL1 to TUBULIN was calculated. The data are presented as mean ± SEM. ***P < 0.001; *P < 0.05; NS, no significance.

Acknowledgments

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