Figure 4

Figure 4

Comparison of DEGs during kras^G12V-induced HCC development and PH-induced liver regeneration in zebrafish. (A) Workflow of the differential expression analysis based on the RNA-Seq reads. (B) Experimental design for comparing the transcriptomic changes in the kras+ livers with those in the PH livers with the quantification of total DEGs. (C,D) Venn diagrams showing the overlaps of (C) upregulated genes and (D) downregulated genes between the kras+ male liver on Day 5 and the PH male liver on Day 1. (E,F) Heatmaps of genes that were significantly (A) upregulated and (B) downregulated in both the kras+ liver on Day 5 and the PH liver on Day 1 based RNA-seq data. (G,H) Expression of (G) fgf13b and (H) s100a1 in the kras+ male livers 24 h after PH/sham surgery as determined by RT-qPCR (n = 4). ns p > 0.05, ** p ≤ 0.01.