Figure 4

Figure 4

VD regulates lipid metabolism in zebrafish under short-term fasting conditions. (A–C) After the last feeding, zebrafish fed with VD₃ or non-VD₃ diet were fasted for 24 h before sampling. The gene expression of ppara, pgc1a, pparg, fasn (A) (n = 4/group), and FFA absorption-related genes cd36, slc27a2a (B) (n = 5/group) in the liver were determined. The gene expressions of ppara, pgc1a, fasn in the dorsal muscle were analyzed (C) (n = 6/group). (D–F) WT and cyp2r1^−/− zebrafish at 3 mpf were fasted for 24 h before sampling. The gene expressions of ppara, pgc1a, pparg, fasn (D) (n = 4/genotype), and cd36, slc27a2a (E) (n = 4/genotype) in the liver were determined. The gene expressions of ppara, pgc1a, fasn in the dorsal muscle were determined (F) (n = 4/genotype). * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no statistical significance.