ZFIN Image: Du et al., 2024, Figure 2

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Figure 2

1,25(OH)₂D₃ generation in zebrafish was impaired under short-term fasting condition. (A–C). The gene expression of cyp2r1 (A), cyp27b1 (B) and cyp24a1 (C) in zebrafish liver was assessed after fasting for 24 h or 48 h, compared to the control group (1 h postprandial) (n = 4/group). (D) The serum 1,25(OH)₂D₃ concentrations were determined (n = 3 replicates, 4~5 fish/replicate). (E,F) Gene expression levels of vdra (E) and vdrb (F) in the liver (n = 4/group). * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no statistical significance.

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