Figure 8

Figure 8

AHR inhibition curtails EDA-mediated AHR target gene upregulation in vitro and in vivo. Bar graphs show the gene expression levels detected by qPCR on RNA obtained from SH-SY5Y cells (a) and zebrafish larvae (b). Cells were treated with DMSO, 30 µM or 100 µM EDA and/or 1 µM GNF-351 for 24 h. (b) Zebrafish larvae at 8 hpf were treated with DMSO or 30 µM EDA in the presence or absence of 1 µM GNF-351 for 24 h and 48 h. The mean ± SEM of 3 experiments is shown. * p < 0.05, ** p < 0.01 and *** p < 0.001 with unpaired Student’s t-test. The image was edited using BioRender.com (https://www.biorender.com/ accessed on 3 April 2024).